Multiple genes of mevalonate and non-mevalonate pathways contribute to high aconites content in an endangered medicinal herb, Aconitum heterophyllum Wall

Phytochemistry
Nikhil MalhotraRajinder Singh Chauhan

Abstract

Aconitum heterophyllum Wall, popularly known as Atis or Patis, is an important medicinal herb of North-Western and Eastern Himalayas. No information exists on molecular aspects of aconites biosynthesis, including atisine- the major chemical constituent of A. heterophyllum. Atisine content ranged from 0.14% to 0.37% and total alkaloids (aconites) from 0.20% to 2.49% among 14 accessions of A. heterophyllum. Two accessions contained the highest atisine content with 0.30% and 0.37% as well as the highest alkaloids content with 2.22% and 2.49%, respectively. No atisine was detected in leaves and shoots of A. heterophyllum, thereby, suggesting that the biosynthesis and accumulation of aconite alkaloids occur mainly in roots. Quantitative expression analysis of 15 genes of MVA/MEP pathways in roots versus shoots, differing for atisine content (0-2.2 folds) showed 11-100 folds increase in transcript amounts of 4 genes of MVA pathway; HMGS, HMGR, PMK, IPPI, and 4 genes of MEP pathway; DXPS, ISPD, HDS, GDPS, respectively. The overall expression of 8 genes decreased to 5-12 folds after comparative expression analysis between roots of high (0.37%) versus low (0.14%) atisine content accessions, but their relative transcript amounts remained...Continue Reading

References

Jul 31, 1992·Biochemical and Biophysical Research Communications·L GondetT J Bach
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·B M LangeR Croteau
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·L M LoisA Boronat
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·B M LangeR Croteau
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J H Julliard, R Douce
Oct 1, 2003·Journal of Biosciences·Vinod Shanker DubeyRajesh Luthra
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Peter Hedden, Yuji Kamiya
Nov 13, 2004·Genome Biology·Jon A Friesen, Victor W Rodwell
Dec 25, 2004·Plant Physiology·Pablo LeivarXavier Fernàndez-Busquets
Jan 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Natalia DudarevaJonathan Gershenzon
Sep 27, 2005·Journal of Pharmaceutical and Biomedical Analysis·Zhaohong WangYe He
Nov 18, 2005·Progress in Lipid Research·Florence BouvierBilal Camara
Jan 1, 1933·The Biochemical Journal·B T Cromwell
Jul 11, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Muhammad NisarM Iqbal Choudhary
Apr 14, 2009·Journal of Experimental Botany·Masashi SuzukiNoriko Nagata
Jun 1, 2006·Neuropsychiatric Disease and Treatment·Martha SajatovicMatthew A Fuller
Jul 9, 2009·Journal of Experimental Botany·Elizabeth CordobaPatricia León
Mar 11, 2011·BMC Plant Biology·Linda OlofssonPeter E Brodelius
Aug 27, 2011·Journal of Experimental Botany·Juri BattilanaMaria Stella Grando
May 11, 2013·Ancient Science of Life·Manjunath AjanalShradda U Nayak
Feb 20, 2014·Indian Journal of Pharmacology·Satyendra K PrasadSiva Hemalatha

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