PMID: 5906374Mar 1, 1966

Multiple hydrolases in bean leaves (Phaseolus vulgaris L.) and the effect of the halo blight disease caused by Pseudomonas phaseolicola (Burkh.) Dowson

Plant Physiology
K RUDOLPH, M A Stahmann

Abstract

Multiple forms of hydrolytic enzymes were demonstrated in extracts of healthy bean leaves (Phascolus vulgaris L.) and bean leaves infected with the halo blight organism [Pseudomonas phaseolicola (Burkh.) Dowson] by polyacrylamide disc electrophoresis. Bean leaves contained up to 4 acid phosphatase bands, 9 esterase bands active towards alpha-naphthyl acetate, and 7 esterase bands towards alpha-naphthyl butyrate. Only low or no activity was found for alkaline phosphatase, lipase, and aminopeptidase. Two artifacts are described which were observed with the lead phosphate method for acid phosphatase and the Tween method for demonstration of lipase. After infection with the halo blight organism the major acid phosphatase of the host increased during early and decreased at later infection stages. An acid phosphatase of bacterial origin with a more neutral pH optimum could be demonstrated in infected leaves. It is suggested that the bacterial acid phosphatase plays a role in uptake of metabolites by the pathogen. Several esterase bands decreased after infection. One host band with activity towards alpha-naphthyl butyrate increased. Also the pathogen showed an esterase band with high activity towards alpha-naphthyl butyrate.

References

Jan 1, 1959·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C L MARKERT, R L HUNTER

Citations

Feb 1, 1980·Biochemical Genetics·M P TimkoD E Fairbrothers
Dec 17, 2011·The Protein Journal·T SubramaniK R Siddalinga Murthy
Feb 1, 1969·Plant Physiology·B H McCownG E Beck
Feb 27, 2007·Bioresource Technology·Afaf S FahmySaleh A Mohamed
Apr 1, 1976·Journal of the Science of Food and Agriculture·P SpettoliG Ferrari
Oct 1, 1974·Journal of the Science of Food and Agriculture·A J FaruqueJ C Hawke

Related Concepts

Hydrolase
Pseudomonas

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