PMID: 11927666Apr 3, 2002Paper

Multiple inhibitory effects of Au(CN)(2-) ions on cystic fibrosis transmembrane conductance regulator Cl(-) channel currents

The Journal of Physiology
Paul Linsdell, Xiandi Gong

Abstract

Lyotropic pseudohalide anions are potentially useful as high affinity probes of Cl(-) channel pores. However, the interaction between these pseudohalides and the cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel have not been described in detail. Here we show that Au(CN)(2-) ions applied to the intracellular face of membrane patches from stably transfected baby hamster kidney cells inhibit CFTR channel currents by at least two mechanisms, which can be distinguished at the single channel level or by inhibiting channel closure using 2 mM pyrophosphate. Low concentrations (< 10 microM) of Au(CN)(2-) significantly reduced CFTR channel open probability. This effect was apparently voltage insensitive, independent of extracellular Cl(-) concentration, and lost following exposure to pyrophosphate. Higher concentrations of intracellular Au(CN)(2-) caused an apparent reduction in unitary current amplitude, presumably due to a kinetically fast blocking reaction. This effect, isolated following exposure to pyrophosphate, was strongly voltage dependent (apparent K(d) 61.6 microM at -100 mV and 913 microM at +60 mV). Both the affinity and voltage dependence of block were highly sensitive to extracellular Cl(-) concentr...Continue Reading

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Citations

Apr 22, 2009·The Journal of Membrane Biology·Mohammad Fatehi, Paul Linsdell
Jul 29, 2011·The Journal of Membrane Biology·Ryan G HolsteadPaul Linsdell
May 2, 2008·Pflügers Archiv : European journal of physiology·Jing-Jun ZhouPaul Linsdell
Nov 2, 2002·British Journal of Pharmacology·Xiandi GongPaul Linsdell
Oct 31, 2002·The Journal of General Physiology·Zhen ZhouTzyh-Chang Hwang
Nov 12, 2003·The Journal of General Physiology·Xiandi Gong, Paul Linsdell
Oct 29, 2003·The Journal of General Physiology·Zhiwei CaiDavid N Sheppard
Jul 16, 2011·International Journal of Nanomedicine·J McCarthyMw Radomski
Mar 29, 2014·Pflügers Archiv : European journal of physiology·Paul Linsdell
May 19, 2009·Canadian Journal of Physiology and Pharmacology·Jing-Jun Zhou, Paul Linsdell
Dec 12, 2003·Molecular Membrane Biology·T S Scott-WardD N Sheppard
Apr 8, 2003·The Journal of Physiology·Xiandi Gong, Paul Linsdell
Jul 14, 2006·Biochimica Et Biophysica Acta·Sergey MissanTerence F McDonald
Jan 12, 2012·Biochimica Et Biophysica Acta·Wuyang Wang, Paul Linsdell
Feb 13, 2015·The Journal of Physiological Sciences : JPS·Hussein N Rubaiy, Paul Linsdell
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Zhiwei CaiDavid N Sheppard
Jun 29, 2007·American Journal of Physiology. Cell Physiology·Brenna L vanTolElizabeth A Cowley
Jan 15, 2021·Biochimica Et Biophysica Acta. Biomembranes·Paul Linsdell

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