Multiple isoforms of starch branching enzyme-I in wheat: lack of the major SBE-I isoform does not alter starch phenotype

Functional Plant Biology : FPB
Ahmed ReginaMatthew K Morell

Abstract

The role of starch branching enzyme-I (SBE-I) in determining starch structure in the endosperm has been investigated. Null mutations of SBE-I at the A, B and D genomes of wheat were identified in Australian wheat varieties by immunoblotting. By combining individual null mutations at the B and D genomes through hybridisation, a double-null mutant wheat, which lacks the B and D isoforms of SBE-I, was developed. Wheat mutants lacking all the three isoforms of SBE-I were generated from a doubled haploid progeny of a cross between the BD double-null mutant line and a Chinese Spring (CS) deletion line lacking the A genome isoform. Comparison of starch from this mutant wheat to that from wild type revealed no substantial alteration in any of the structural or functional properties analysed. Further analysis of this triple-null mutant line revealed the presence of another residual peak of SBE-I activity, referred to as SBE-Ir, in wheat endosperm representing < 3% of the activity of SBE-I in wild type endosperm.

References

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Citations

Aug 28, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Christine Konik-RoseZhongyi Li
Jul 28, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Elizabeth S DennisW J Peacock
May 12, 2016·Cellular and Molecular Life Sciences : CMLS·Barbara Pfister, Samuel C Zeeman
Oct 31, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jian XiaYueming Yan
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ahmed ReginaMatthew Morell
Jul 15, 2020·Biomolecules·Henrike BrustJoerg Fettke
Jan 5, 2013·Journal of the Science of Food and Agriculture·Jody E HigginsSadequr Rahman
Apr 20, 2017·Frontiers in Plant Science·Juan WangCunxu Wei
Sep 27, 2020·International Journal of Molecular Sciences·Ian J Tetlow, Eric Bertoft
Mar 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Haiteng LiSushil Dhital
Sep 30, 2014·Carbohydrate Polymers·Alex Chi WuRobert G Gilbert
Jul 28, 2021·The Plant Journal : for Cell and Molecular Biology·Qin ZhangJean-Philippe F Ral

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Methods Mentioned

BETA
electrophoresis
affinity
scanning electron micrography
differential scanning calorimetry
PCR

Software Mentioned

Genstat
UVI Photo
Thermocline

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