PMID: 9548563Apr 21, 1998Paper

Multiple levels of steroid hormone-dependent control of osteocalcin during osteoblast differentiation: glucocorticoid regulation of basal and vitamin D stimulated gene expression

Journal of Cellular Biochemistry
V ShalhoubJ B Lian

Abstract

We have examined the contribution of transcriptional mechanisms to the pleiotropic effects of glucocorticoids on basal and vitamin D stimulated expression of the developmentally regulated bone-specific osteocalcin (OC) gene. OC expression was systematically investigated at the level of protein, mRNA, and newly synthesized transcripts during maturation of the bone cell phenotype in cultures of fetal rat calvarial-derived osteoblasts. Our results indicate that transcriptional control of basal and hormone-regulated OC expression predominates in immature osteoblasts prior to matrix mineralization. However, in mature osteoblasts OC expression is controlled primarily by posttranscriptional mechanisms reflected by elevated mRNA levels with a decline in transcription. Vitamin D, alone or in combination with Dex, is a significant factor contributing to mRNA stabilization in mature osteoblasts with a mineralized extracellular matrix. Transcriptional modifications in response to Dex are reflected by quantitative differences between proliferating and mature osteoblasts in the formation of glucocorticoid receptor binding complexes at the proximal OC glucocorticoid response element. Vitamin D and glucocorticoid receptor mRNA levels are signi...Continue Reading

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Citations

Nov 20, 2012·Tissue Engineering. Part a·Núria Marí-BuyéCarlos E Semino
Apr 6, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J A RobinsonT C Spelsberg
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Nov 10, 2012·Journal of Cellular Physiology·Anna NeveFrancesco Paolo Cantatore
Aug 19, 2007·Biochemical and Biophysical Research Communications·Anurag GuptaDietmar Werner Hutmacher
Jul 18, 2000·Experimental Cell Research·G P ThomasE M Gardiner
Jul 13, 2002·Journal of Biomedical Materials Research·François CornetJoseph Jeanfils
Feb 15, 2003·Molecular Endocrinology·Michael J CoghlanJeffrey N Miner

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