Multiple-locus variable-number tandem repeat analysis for subtyping of Salmonella enterica subsp. enterica serovar Enteritidis

International Journal of Medical Microbiology : IJMM
Andreas BeranekChristian Kornschober

Abstract

Salmonella enterica subsp. enterica serovar Enteritidis is a major food-borne pathogen that caused most of Salmonella infections worldwide. S. Enteritidis phage type 4 (PT4) especially presents a real challenge for the classical typing methods. We developed a simple multiple-locus variable-number tandem repeat analysis (MLVA) assay based on three hypervariable variable-number tandem repeat (VNTR) loci for subtyping of Salmonella Enteritidis. Testing an arbitrary chosen strain collection of 110 S. Enteritidis isolates, comprising PTs 4, 8, and 21, the MLVA assay yielded a higher discriminatory power, corresponding to a Simpson's index of diversity (ID) of 0.91, when compared to pulsed-field gel electrophoresis (PFGE) which had a Simpson's ID of 0.41. To simplify interpretation of results, we developed a VNTR allele code based on the repeat unit number. This code can easily be exchanged. In conclusion, MLVA is a promising new tool to investigate outbreaks of S. Enteritidis and constitutes a useful addition to the current phage typing scheme.

Citations

Nov 24, 2011·Avian Pathology : Journal of the W.V.P.A·Min S KangGab S Chung
Aug 23, 2011·Applied and Environmental Microbiology·Pierre WattiauSophie Bertrand
Jun 19, 2009·Journal of Clinical Microbiology·Sophie Octavia, Ruiting Lan
Sep 7, 2013·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·C A NadonUNKNOWN MLVA Harmonization Working Group
Aug 27, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M-F PhobaJ Jacobs
Apr 9, 2013·International Journal of Food Microbiology·Lisa BarcoAntonia Ricci
Mar 13, 2009·Wiener klinische Wochenschrift·Christian KornschoberBurkhard Springer
Oct 22, 2013·Diagnostic Microbiology and Infectious Disease·Soo Tein Ngoi, Kwai Lin Thong
Mar 20, 2010·Current Opinion in Biotechnology·Dave Boxrud
Mar 10, 2017·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Tansy PetersElizabeth de Pinna
Jul 2, 2010·Electrophoresis·Gi Won ShinGyoo Yeol Jung
Jul 30, 2019·Frontiers in Microbiology·Silin TangMartin Wiedmann

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