Multiple m6A RNA methylation modulators promote the malignant progression of hepatocellular carcinoma and affect its clinical prognosis.

BMC Cancer
Nanfang QuHaixing Jiang

Abstract

Hepatocellular carcinoma (HCC) is the second most common cause of cancer-related death in the world. N6-methyladenosine (m6A) RNA methylation is dynamically regulated by m6A RNA methylation modulators ("writer," "eraser," and "reader" proteins), which are associated with cancer occurrence and development. The purpose of this study was to explore the relationships between m6A RNA methylation modulators and HCC. First, using data from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) databases, we compared the expression levels of 13 major m6A RNA methylation modulators between HCC and normal tissues. Second, we applied consensus clustering to the expression data on the m6A RNA methylation modulators to divide the HCC tissues into two subgroups (clusters 1 and 2), and we compared the clusters in terms of overall survival (OS), World Health Organization (WHO) stage, and pathological grade. Third, using least absolute shrinkage and selection operator (LASSO) regression, we constructed a risk signature involving the m6A RNA methylation modulators that affected OS in TCGA and ICGC analyses. We found that the expression levels of 12 major m6A RNA methylation modulators were significantly different betwee...Continue Reading

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Citations

Sep 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yueqin ZhuYan Huang
Mar 12, 2021·Frontiers in Oncology·Yirong XuQing Guo
Mar 19, 2021·Cancer Cell International·Wei ZhuChen Lu
Apr 3, 2021·Cell Death & Disease·Rongkai ShiHongchuan Jin
May 7, 2021·Cancer Communications·Shenghua DongHuilin Huang

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Methods Mentioned

BETA
PCA

Software Mentioned

Perl
Hmisc
R
limma
ActivePerl
ggplot2
R package “ pheatmap
Formula
SPSS
lattice

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