PMID: 8604318Feb 15, 1996Paper

Multiple mechanisms may contribute to the cellular anti-adhesive effects of phosphorothioate oligodeoxynucleotides

Nucleic Acids Research
Z KhaledC A Stein

Abstract

Phosphorothioate oligodeoxynucleotides complementary to the p65 (Rel A) subunit of the NF-kappaB nuclear transcriptional regulatory factor have been suggested to be sequence specific blockers of cellular adhesion. We studied the effects of Rel A antisense, Rel A sense and other phosphorothioate oligodeoxynucleotides on cellular adhesion and found that blockade of adhesion was predominately non-sequence specific. Phosphorothioate oligodeoxynucleotides bind to the extracellular matrix (ECM) of NIH 3T3 cells, and to the ECM elements laminin and fibronectin. By use of a gel mobility shift assay, the association of the A subunit of laminin with a probe 12mer phosphodiester oligodeoxynucleotide could be demonstrated. This interaction was described by a single-site binding equation (K d = 14 microM). Human Rel A antisense and sense oligodeoxynucleotides, and two synthetic persulfated heparin analogs were excellent competitors of the binding of the probe oligodeoxynucleotide to laminin. Taken together, these data indicate that oligodeoxynucleotide binding occurred at or near the heparin-binding site. Competition for 5' 32p- SdT18 (an 18mer phosphorothioate homopolymer of thymidine) binding to fibronectin with the discrete heparin analo...Continue Reading

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Citations

Jul 26, 2008·Journal of Bioenergetics and Biomembranes·C A Stein, Marco Colombini
Apr 6, 2004·Current Opinion in Pharmacology·Ryuichi MorishitaToshio Ogihara
Jul 13, 2001·Pharmacology & Therapeutics·C J Wraight, P J White
Dec 1, 2001·Pharmacology & Therapeutics·R MorishitaT Ogihara
Nov 26, 2002·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Nathalie Dias, C A Stein
Apr 3, 1999·British Journal of Pharmacology·J PanésD N Granger
Feb 6, 2003·Antisense & Nucleic Acid Drug Development·Maria MaszewskaMaria Koziołkiewicz
Apr 1, 1997·Antisense & Nucleic Acid Drug Development·W WangL E Rabbani
Aug 1, 1997·Antisense & Nucleic Acid Drug Development·T E Saison-BehmoarasC Hélène
Apr 7, 1999·Antisense & Nucleic Acid Drug Development·A W Szklarczyk, L Kaczmarek
Feb 28, 2002·Annual Review of Immunology·Arthur M Krieg
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·I Lebedeva, C A Stein
Dec 25, 2002·Circulation Journal : Official Journal of the Japanese Circulation Society·Ryuichi Morishita
Nov 7, 1998·Clinical Chemistry and Laboratory Medicine : CCLM·R MorishitaT Ogihara
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·I V Lebedeva, C A Stein
Jul 5, 2006·International Reviews of Immunology·Heather L WilsonGeorge K Mutwiri
Mar 25, 2000·The Journal of Investigative Dermatology·H Schlagbauer-WadlB Jansen
Mar 30, 2004·Clinical Prostate Cancer·Luba Benimetskaya, C A Stein
Aug 27, 1998·Biochemical Pharmacology·S Corrias, Y C Cheng
Aug 24, 1999·Methods : a Companion to Methods in Enzymology·R B RunyanD L Weeks
Sep 7, 2001·The Journal of Clinical Investigation·C A Stein
Apr 30, 2003·Antiviral Chemistry & Chemotherapy·Paul F Torrence, Linda D Powell
Nov 24, 1999·The Journal of Laboratory and Clinical Medicine·D E Macfarlane, L Manzel
Dec 22, 1999·Oncogene·B Rayet, C Gélinas
Jun 19, 2018·Annals of Medicine·Irene Marafini, Giovanni Monteleone
Jan 16, 2003·Oncogene·Robert ElezStefan Zeuzem
Sep 2, 2004·Molecular Pharmacology·Pilar BlancafortCarlos F Barbas
Sep 5, 2014·Current Opinion in Oncology·Daniela Castanotto, Cy A Stein
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tara L RobertsKatryn J Stacey
Jun 6, 2020·Frontiers in Pharmacology·Cheng-Kai HuangThomas Thum

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