Multiple mechanisms regulate expression of low temperature responsive (LOT) genes in Saccharomyces cerevisiae

Biochemical and Biophysical Research Communications
L ZhangR Imai

Abstract

Using cDNA subtraction screening, we identified five Saccharomyces cerevisiae genes whose expressions is up-regulated when culture temperature was down-shifted from 30 to 10 degrees C. Among these LOT (low temperature-responsive) genes, three (LOT1, LOT2, and LOT3) were identical to FBA1, RPL2B, and NOP1, encoding a fructose biphosphate aldolase, a ribosomal protein L2B, and a nucleolar protein for rRNA processing, respectively. No functions were assigned for LOT5 and LOT6, which are identical to YKL183w and YLR011w, respectively. Northern hybridization analysis revealed that these genes are not uniformly regulated in response to the change of growth temperature. In addition, all the LOT genes, except for LOT1/FBA1, were induced by a low concentration of cycloheximide. The data indicate that multiple mechanisms, including translational functionality may be involved in the regulation of LOT gene expression in yeast.

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Citations

Jul 17, 2008·Comparative and Functional Genomics·M BecerraM E Cerdán
Jun 17, 2004·Science's STKE : Signal Transduction Knowledge Environment·Masayori Inouye, Sangita Phadtare
Dec 18, 2008·Extremophiles : Life Under Extreme Conditions·Yana G GochevaMaria B Angelova
Jul 16, 2003·Biochemical and Biophysical Research Communications·Lei ZhangAkinori Ohta
Feb 15, 2007·FEMS Microbiology Reviews·Jaime AguileraJose Antonio Prieto
Jul 25, 2007·FEMS Microbiology Reviews·Brian R GibsonKatherine A Smart
Jul 28, 2012·FEMS Yeast Research·Rosana ChivaJósé Manuel Guillamón
Oct 7, 2004·Canadian Journal of Microbiology·Silvia M Díaz PradoM Isabel González Siso
Sep 19, 2001·Yeast
Jun 24, 2006·The Biochemical Journal·Mohamed B Al-Fageeh, C Mark Smales

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