Multiple Menkes copper ATPase (Atp7a) transcript and protein variants are induced by iron deficiency in rat duodenal enterocytes.

Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)
Yan LuJames F Collins

Abstract

The Menkes copper ATPase (Atp7a) pumps copper into the trans-Golgi for cuproenzyme synthesis, and translocates to the basolateral membrane of enterocytes for copper export. Recent studies demonstrated that three 5' end splice variants of the Atp7a transcript exist in rat duodenum, all of which are strongly induced during iron deprivation. To explore a possible role for Atp7a (and copper) in intestinal iron absorption, the current studies were undertaken to test the hypothesis that multiple Atp7a transcript and protein variants exist in intestinal epithelial cells. Northern blot analyses using probes generated from the full-length Atp7a cDNA revealed several specific hybridization bands, all of which were more intense in RNA samples extracted from duodenal enterocytes isolated from iron-deficient rats. A PCR-based approach, using forward primers specific for the alternative 5' end splice variants and a reverse primer in exon 23, demonstrated that 3 full-length transcripts exist in rat IEC-6 cells. To identify possible Atp7a protein variants, three distinct polyclonal antisera were utilized. The specificity of the antisera was first established by western blotting and immunoprecipitation studies using samples derived from isolate...Continue Reading

References

Jan 10, 1998·The Biochemical Journal·M L AcklandJ F Mercer
Jan 8, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·James F CollinsFayez K Ghishan
Feb 9, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·James F CollinsRichard W Browne
Aug 15, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·James F CollinsP N Ranganathan
Aug 20, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lingli JiangJames F Collins
Feb 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Perungavur N RanganathanJames F Collins
Feb 22, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Perungavur N RanganathanJames F Collins

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Citations

Nov 24, 2012·American Journal of Physiology. Cell Physiology·Liwei Xie, James F Collins

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