PMID: 3936834Nov 1, 1985

Multiple modes of inhibition of spore germination and outgrowth by reduced pH and sorbate

The Journal of Applied Bacteriology
J C Blocher, F F Busta

Abstract

Germination and outgrowth of three strains of Clostridium botulinum in PYEG medium were measured by phase contrast microscopy. Reduction in pH from 7 to 5.5 completely inhibited germination of strain 12885A, reduced the extent of germination of strain 62A and had no effect on the extent of germination of strain 53B. At pH 5.5, 225 mg/l of undissociated sorbic acid had no effect on the germination of strain 53B, while at pH 6.5, 225 mg/l of undissociated sorbic acid completely inhibited germination of strains 62A and 12885A. Outgrowth of germinated spores of strains 62A and 53B was not inhibited at pH 5.5, but the addition of sorbate (225 mg/l undissociated sorbic acid) completely inhibited outgrowth. Sorbate inhibited germination of Cl. botulinum and Bacillus cereus spores triggered to germinate by amino acids. Inhibition occurred after germinant binding, as measured by commitment to germinate.

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Citations

Mar 24, 2010·Applied Microbiology and Biotechnology·Zhenhua HuoQirong Shen
Sep 17, 1999·International Journal of Food Microbiology·S Brul, P J Coote
Feb 1, 2011·Applied and Environmental Microbiology·C C J van MelisTjakko Abee
Sep 15, 2004·International Journal of Food Microbiology·M Del TorreM W Peck
Mar 21, 2015·Food Microbiology·Maryam AlnomanMahfuzur R Sarker
Jan 14, 2004·Microbiological Research·Han-Chul KangSeung-Joo Go
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Aug 10, 2017·Microorganisms·Lucille GarnierJérôme Mounier
Jul 20, 2016·Journal of Bacteriology·Disha BhattacharjeeJoseph A Sorg
May 3, 2019·Nutrition and Cancer·Fardin JavanmardiAmin Mousavi Khaneghah

Related Concepts

Bacillus cereus
Clostridium botulinum
Fatty Acids, Unsaturated
Hydrogen-Ion Concentration
Sodium Sorbate
Spores, Bacterial

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