Multiple molecular forms of human pancreas alanine aminopeptidase

Clinica Chimica Acta; International Journal of Clinical Chemistry
W SidorowiczF J Behal

Abstract

Human pancreas is the source of an alanine aminopeptidase (HPAA) that is unique to pancreas and is readily distinguishable from the liver, kidney, and duodenal alanine aminopeptidases. Each of these three aminopeptidases appears in small quantities in blood and therefore may consitute tissue/organ specific marker enzymes. In this study alanine aminopeptidase from pancreas has been purified. Pancreas alanine aminopeptidase was resolved upon purification with ion exchange chromatography into three isoenzymes. Gel filtration chromatography of these isoenzymes indicates that their molecular weights are near 235 000 daltons. The isoenzymes contain a firmly bound divalent cation that could be removed with EDTA only at temperatures above 40 degrees C (at which the enzymes were stable) and below 52 degrees C (at which thermal denaturation begins to take place). Treatment with EDTA at 4 degrees C yields fully active enzymes, which, however may be stimulated approximately 200% by the addition of Co2+ at 10(-4) mol/l. This cobalt stimulation is easily reversed by dialysis of the stimulated isoenzymes against deionized water. The pancreas alanine aminopeptidases were not inhibited by tosyl-phenylalanine chloromethylketone or by tosylleucin...Continue Reading

References

Aug 1, 1977·Archives of Biochemistry and Biophysics·C W Garner, F J Behal
Jun 21, 1976·Archives of Dermatological Research·W P Herrmann
Jul 15, 1975·Biochemistry·C W Garner, F J Behal
Jul 30, 1974·Biochemistry·C W Garner, F J Behal
Aug 27, 1971·Biochimica Et Biophysica Acta·G H Little, F J Behal
Mar 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·G H Little, F J Behal
Apr 1, 1969·Archives of Biochemistry and Biophysics·F J Behal, M N Story
Aug 1, 1965·Archives of Biochemistry and Biophysics·F J BehalJ Hardman
Jun 1, 1964·Cancer·A M RUTENBURGJ A GOLDBARG
Nov 1, 1964·Archives of Biochemistry and Biophysics·F J BEHALL C TERRELL

❮ Previous
Next ❯

Citations

Mar 19, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·W SidorowiczF J Behal
Jan 1, 1986·The International Journal of Biochemistry·Y Agrawal, T Vanha-Perttula
Mar 1, 1994·Comparative Biochemistry and Physiology. Biochemistry and Molecular Biology·I Skrtić, L Vitale
Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hironori UmetsuKazuo Sasaki
Jul 6, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·S ElgünS Demirtaş
Dec 1, 1988·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·G J SanderinkG Siest
Jan 8, 2008·Journal of Molecular Biology·Manuel MorillasEva Kuennemann
Nov 6, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·W SidorowiczF J Behal
Sep 26, 1986·Biochimica Et Biophysica Acta·K LaluT Vanha-Perttula

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.