Multiple mutant analysis of recombination in yeast.

Molecular & General Genetics : MGG
R E Malone

Abstract

Summary. The RAD6, RAD50, and RAD52 loci have been identified as genes which code for functions which may act during meiotic recombination in yeast (Game et al.1980; Prakash et al. 1980). By use of the spol3-1 mutation,which allows sporulating cells to bypass the first meiotic division, the rad50-1 mutation has been directly implicated as a general meiotic Rec- mutation by examination of viable ascospores (Malone and Esposito 1981). Since the rad6-1 and rad52-1 mutations do not yield viable ascospores in the presence of spol3-1, multiple rad mutants have been constructed and analyzed. This analysis has demonstrated that in meiosis tad50-1 is epistatic to rad52-1, and rad6-1 is epistatic to rad50-1. This suggests that the order of action of these genes during meiosis is RAD6, RAD50, and then RAD52. The data for rad6-1 can be interpreted to suggest that RAD6 may not code for a recombination function,per se, although it may be required for recombination to occur. Analysis of mitotic recombination indicates that rad52-1 is epistatic to rad50-1 ; in mitosis; this is consistent with the hypothesis that the RAD50 gene codes for a recombination function required in meiosis but not in mitosis.

References

Jan 1, 1976·Annual Review of Genetics·B S BakerL Sandler
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M S Meselson, C M Radding
Apr 15, 1974·Journal of Molecular Biology·L M Hereford, L H Hartwell
Jul 1, 1966·Genetics·M DemerecP E Hartman
Jul 23, 1981·Nature·J A Jackson, G R Fink
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R E Malone, R E Esposito
Jan 1, 1980·Chromosoma·K L Satya-PrakashS Pathak
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R K MortimerD Schild
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J E WagstaffR E Esposito
Oct 1, 1981·Molecular and Cellular Biology·R E Malone, R E Esposito
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J N StrathernI Herskowitz
Apr 1, 1980·Current Genetics·R E MaloneM S Esposito
Jul 1, 1981·Current Genetics·L Prakash, P Taillon-Miller

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Citations

Jan 1, 1992·Developmental Genetics·C N Giroux
Aug 1, 1995·Current Genetics·O V ChepurnayaV G Korolev
Aug 1, 1989·Molecular & General Genetics : MGG·E A Thompson, G S Roeder
Aug 1, 2000·Mutation Research·J C Game
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·S T MerinoM E Zolan
Nov 29, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lorraine S Symington
Aug 13, 2005·Progress in Nucleic Acid Research and Molecular Biology·S Anuradha, K Muniyappa
Sep 25, 1998·Critical Reviews in Biochemistry and Molecular Biology·K Muniyappa, K M Kironmai
Apr 5, 1996·The Journal of Biological Chemistry·G J MagesE L Winnacker
Sep 30, 1995·Yeast·M D Kaytor, D M Livingston
Sep 1, 1990·Molecular & General Genetics : MGG·J R Simon, P D Moore
May 5, 1998·Molecular and Cellular Biology·K A Farr, M A Hoyt
Nov 4, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Min ZhangWei Xiao
Jan 6, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Min ZhangWei Xiao
Mar 1, 1991·Molecular and Cellular Biology·B A Ozenberger, G S Roeder

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