Multiple mutations in the acetylcholinesterase 3 gene associated with organophosphate resistance in Rhipicephalus (Boophilus) microplus ticks from Punjab, India

Veterinary Parasitology
JyotiS S Rath

Abstract

The organophosphate (OP) resistance status in Rhipicephalus (Boophilus) microplus ticks collected from seventeen districts located in the northwestern Indian state, Punjab were characterized using three data sets (bioassay, biochemical and molecular assays). Adult immersion test (AIT) was adopted and the resistance factors (RF) for the field isolates were determined. Resistance to malathion was detected in 12 isolates among which 11 showed level I resistance status while level II status was recorded in one isolate (RF of 5.35). To understand the possible mechanism of resistance development, acetylcholinesterase (AChE) activity and gene sequences of the AChE3 were analyzed. A significantly (P<0.001) higher level of percent uninhibited AChE activity was recorded in all field isolates (36.36±0.46-43.77±1.21) in comparison to the susceptible population (29.39±0.40). The AChE activity was positively correlated with RF against malathion with a correlation coefficient (r) of 0.359. Analysis of nucleotides and their deduced amino acids sequences of partial AChE3 gene revealed the presence of six amino acid substitutions (I48L, I54V, V71A, I77M, S79P and R86Q). Three novel amino acid substitutions (V71A, I77M and S79P) in partial AChE3 ...Continue Reading

References

Oct 23, 2013·Asian Pacific Journal of Tropical Medicine·Nirbhay Kumar Singh, Shitanshu Shekhar Rath
Oct 17, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology· JyotiS S Rath

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Citations

Sep 27, 2018·International Journal of Veterinary Science and Medicine·O E Ola-DaviesM O Abatan
Jun 2, 2017·Ticks and Tick-borne Diseases·Luciana Gatto BritoMárcia Cristina de Sena Oliveira

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