PMID: 26807199Jan 26, 2016Paper

Multiple myeloma cell lines and primary tumors proteoma: protein biosynthesis and immune system as potential therapeutic targets

Genes & Cancer
Rodrigo Carlini FernandoGisele W B Colleoni

Abstract

Despite great advance in multiple myeloma (MM) treatment since 2000s, it is still an incurable disease and novel therapies are welcome. Therefore, the purpose of this study was to explore MM plasma cells' (MM-PC) proteome, in comparison with their normal counterparts (derived from palatine tonsils of normal donors, ND-PC), in order to find potential therapeutic targets expressed on the surface of these cells. We also aimed to evaluate the proteome of MM cell lines with different genetic alterations, to confirm findings obtained with primary tumor cells. Bone marrow (BM) samples from eight new cases of MM and palatine tonsils from seven unmatched controls were submitted to PC separation and, in addition to two MM cell lines (U266, RPMI-8226), were submitted to protein extraction for mass spectrometry analyses. A total of 81 proteins were differentially expressed between MM-PC and ND-PC - 72 upregulated and nine downregulated; U266 vs. RPMI 8226 cell lines presented 61 differentially expressed proteins - 51 upregulated and 10 downregulated. On primary tumors, bioinformatics analyses highlighted upregulation of protein biosynthesis machinery, as well as downregulation of immune response components, such as MHC class I and II, and ...Continue Reading

References

Dec 1, 1981·American Journal of Hematology·E H Kraut, A L Sagone
Aug 8, 1998·Seminars in Immunology·M C Carroll
Apr 30, 1999·Leukemia & Lymphoma·G LugassyM Schlesinger
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
Oct 31, 2002·Nature Immunology·Gavin P DunnRobert D Schreiber
Mar 23, 2004·Annual Review of Immunology·Gavin P DunnRobert D Schreiber
Dec 21, 2004·Nucleic Acids Research·Donna MaglottTatiana Tatusova
Mar 17, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Jianbiao ZhouJohn G Gribben
Apr 6, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Philip R GreippJan Westin
Jun 13, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Constantine S MitsiadesKenneth C Anderson
May 4, 2007·The Journal of Clinical Investigation·Jeremy B Swann, Mark J Smyth
Jun 19, 2007·Immunology Letters·Paolo Macor, Francesco Tedesco
Aug 24, 2007·Advances in Experimental Medicine and Biology·Natalia AptsiauriFederico Garrido
Nov 9, 2007·Nature Biotechnology·Daniel Ricklin, John D Lambris
Mar 12, 2008·Blood·Robert A Kyle, S Vincent Rajkumar
Sep 24, 2008·Molecular and Cellular Biology·Leonard B MaggiJason D Weber
Dec 17, 2008·Cancer Chemotherapy and Pharmacology·Flaubert Mbeunkui, Donald J Johann
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jun 25, 2009·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Heiichiro UdonoTakashi Imai
Feb 20, 2010·Medical Oncology·Jacob P LaubachKenneth C Anderson
Apr 27, 2010·Seminars in Cancer Biology·Ben-Zion Katz
Jul 24, 2010·Nature Reviews. Cancer·Jane TrepelLen Neckers
Aug 24, 2010·Molecular Biotechnology·Jana CumovaRoman Hajek
Jan 1, 1844·Medico-chirurgical Transactions·S Solly
Oct 15, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M A Dimopoulos, E Terpos
Jan 8, 2011·Acta Biochimica Et Biophysica Sinica·Feng GeXian'en Zhang
Jun 8, 2012·Current Cancer Drug Targets·Paola Neri, Nizar J Bahlis
Aug 31, 2013·Journal of Leukocyte Biology·Sujatha Muralidharan, Pranoti Mandrekar
Mar 29, 2014·Oncotarget·Lei ZhangFaith E Davies
Mar 7, 2015·Nature Reviews. Drug Discovery·Mamatha BhatIvan Topisirovic

❮ Previous
Next ❯

Citations

Feb 1, 2018·Oncotarget·Angela Isabel Pereira EugênioGisele Wally Braga Colleoni
Sep 19, 2018·International Journal of Molecular Medicine·Huiling MeiTao Guo
Mar 6, 2019·Current Molecular Medicine·Mariana B de OliveiraGisele W B Colleoni
Aug 11, 2020·Journal for Immunotherapy of Cancer·Robyn A A OldhamJeffrey A Medin
Apr 10, 2021·Frontiers in Oncology·Rodrigo Carlini FernandoGisele Wally Braga Colleoni
Jul 22, 2021·Cancer Biology & Therapy·Fabio SallustioAnna Gallone

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.