Multiple Novel Mutations in Plasmodium falciparum Chloroquine Resistance Transporter Gene during Implementation of Artemisinin Combination Therapy in Thailand.

The American Journal of Tropical Medicine and Hygiene
Pattakorn BuppanS Jongwutiwes

Abstract

Mutations in the chloroquine resistance transporter gene of Plasmodium falciparum (Pfcrt) are associated with drug susceptibility status of chloroquine and other antimalarials that interfere with heme detoxification process including artemisinin. We aim to investigate whether an increase in duration of artemisinin combination therapy (ACT) in Thailand could affect mutations in Pfcrt. The complete coding sequences of Pfcrt and dihydrofolate reductase (Pfdhfr), and size polymorphisms of the merozoite surface proteins-1 and 2 (Pfmsp-1 and Pfmsp-2) of 189 P. falciparum isolates collected during 1991 and 2016 were analyzed. In total, 12 novel amino acid substitutions and 13 novel PfCRT haplotypes were identified. The most prevalent haplotype belonged to the Dd2 sequence and no wild type was found. A significant positive correlation between the frequency of Pfcrt mutants and the year of sample collection was observed during nationwide ACT implementation (r = 0.780; P = 0.038). The number of haplotypes and nucleotide diversity of isolates collected during 3-day ACT (2009-2016) significantly outnumbered those collected before this treatment regimen. Positive Darwinian selection occurred in the transmembrane domains only among isolates ...Continue Reading

References

Oct 2, 1965·Lancet·T HarinasutaC Viravan
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·S Thaithong, G H Beale
Aug 23, 2001·The Journal of Infectious Diseases·T E Wellems, C V Plowe
Apr 9, 2002·The Lancet Infectious Diseases·Chansuda WongsrichanalaiSteven R Meshnick
Jun 7, 2003·The Journal of Infectious Diseases·James G KublinChristopher V Plowe
Dec 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Austin L HughesMeredith Yeager
Jul 19, 2005·Proceedings. Biological Sciences·Denae NashTim J C Anderson
Jun 19, 2008·Annals of the New York Academy of Sciences·Austin L Hughes
May 14, 2011·Molecular Ecology Resources·Laurent Excoffier, Heidi E L Lischer
May 21, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C PutaporntipS Jongwutiwes
Oct 18, 2011·Emerging Infectious Diseases·Somchai JongwutiwesChaturong Putaporntip
Oct 2, 2012·Trends in Parasitology·Andrea EckerDavid A Fidock
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 10, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C PutaporntipA L Hughes
Nov 30, 2016·Methods in Molecular Biology·Darren P MartinBrejnev Muhire
Jan 25, 2018·The American Journal of Tropical Medicine and Hygiene·Elizabeth Hemming-SchroederGuiyun Yan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Related Papers

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
S SeethamchaiS Jongwutiwes
Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
C PutaporntipA L Hughes
© 2022 Meta ULC. All rights reserved