Multiple nucleic acid binding sites and intrinsic disorder of severe acute respiratory syndrome coronavirus nucleocapsid protein: implications for ribonucleocapsid protein packaging

Journal of Virology
Chung-Ke ChangTai-Huang Huang

Abstract

The nucleocapsid protein (N) of the severe acute respiratory syndrome coronavirus (SARS-CoV) packages the viral genomic RNA and is crucial for viability. However, the RNA-binding mechanism is poorly understood. We have shown previously that the N protein contains two structural domains--the N-terminal domain (NTD; residues 45 to 181) and the C-terminal dimerization domain (CTD; residues 248 to 365)--flanked by long stretches of disordered regions accounting for almost half of the entire sequence. Small-angle X-ray scattering data show that the protein is in an extended conformation and that the two structural domains of the SARS-CoV N protein are far apart. Both the NTD and the CTD have been shown to bind RNA. Here we show that all disordered regions are also capable of binding to RNA. Constructs containing multiple RNA-binding regions showed Hill coefficients greater than 1, suggesting that the N protein binds to RNA cooperatively. The effect can be explained by the "coupled-allostery" model, devised to explain the allosteric effect in a multidomain regulatory system. Although the N proteins of different coronaviruses share very low sequence homology, the physicochemical features described above may be conserved across differe...Continue Reading

References

Jan 1, 1990·Annual Review of Microbiology·M M Lai
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·B A ShoemakerP G Wolynes
Sep 11, 2002·Structure·Pascal GarciaMarta Bruix
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Aug 15, 2003·Nature·Fabrizio ChitiChristopher M Dobson
Dec 4, 2003·The Journal of General Virology·David KarlinSonia Longhi
Mar 17, 2004·Biochemical and Biophysical Research Communications·Runtao HeXuguang Li
May 19, 2004·Biochemistry·Qiulong HuangEdward T Olejniczak
Jul 17, 2004·Science·Charalampos G KalodimosRobert Kaptein
Jul 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peter Tompa, Peter Csermely
Sep 11, 2004·Biochemical and Biophysical Research Communications·Cheng LuoHualiang Jiang
Jun 1, 2005·Biophysical Journal·Maxim V Petoukhov, Dmitri I Svergun
Oct 18, 2005·Journal of Biomedical Science·Chung-ke ChangTai-huang Huang
Sep 19, 2006·Virology·Sonia ZúñigaLuis Enjuanes
May 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Vincent J Hilser, E Brad Thompson
Nov 24, 2007·Nucleic Acids Research·Roland Ivanyi-NagyJean-Luc Darlix
Feb 5, 2008·Biochemical and Biophysical Research Communications·Yingchun ZengZhenghui Wu

❮ Previous
Next ❯

Citations

Jul 8, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·I Jung ChenMing Hon Hou
May 8, 2009·Journal of Virology·Kelley R HurstPaul S Masters
Jan 15, 2014·Antiviral Research·Chung-ke ChangTai-huang Huang
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Apr 7, 2016·Cell Communication and Signaling : CCS·Aino I JärvelinAlfredo Castello
Jun 8, 2014·Virus Research·Jean-Luc DarlixYves Mély
Mar 5, 2015·Virus Research·Dong Yang, Julian L Leibowitz
May 24, 2016·Virus Research·Samaporn TeeravechyanAnan Jongkaewwattana
May 28, 2016·Cellular and Molecular Life Sciences : CMLS·Sushmita Basu, Ranjit Prasad Bahadur
Aug 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Yong Sheng WangMing Hon Hou
Feb 20, 2016·Acta Crystallographica. Section D, Structural Biology·Nicolas PapageorgiouBruno Coutard
Sep 26, 2020·Frontiers in Molecular Biosciences·Eleni Nikolakaki, Thomas Giannakouros
Jan 16, 2019·Acta Crystallographica. Section D, Structural Biology·Thi Hong Van NguyenBruno Coutard
Jul 28, 2020·Cellular and Molecular Life Sciences : CMLS·Rajanish GiriVladimir N Uversky
Jul 20, 2017·Scientific Reports·Yingying CongFulvio Reggiori
Nov 18, 2020·The EMBO Journal·Theodora Myrto PerdikariNicolas L Fawzi
Dec 3, 2020·PLoS Pathogens·Dhurvas Chandrasekaran DineshEvzen Boura
Sep 18, 2020·Molecular Biology and Evolution·Erica T PratesDaniel Jacobson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.