Multiple pathways for isoleucine biosynthesis in the spirochete Leptospira.

Journal of Bacteriology
H N WestfallD Peterson

Abstract

Spirochetes of the genus Leptospira have previously been shown to use an unusual pathway to synthesize isoleucine. For reasons of convenience, we assume that only one unusual pathway is found in the genus, and we refer to it as the pyruvate pathway. We determined the distribution of this pyruvate pathway in representatives of the seven Leptospira DNA hybridization groups. Our method included labeling the representative strains with radioactive carbon dioxide and other radioactive precursors, fractionating the cells, and determining the specific activities (counts detected per nanomole) of the amino acids found in the protein fractions. On the basis of isoleucine biosynthesis, we found that the genus can be classified as follows: class I primarily, if not exclusively, uses the well-known threonine pathway; class II uses mostly the pyruvate pathway, with a minor amount of isoleucine being synthesized via the threonine pathway; and class III uses the pyruvate pathway exclusively. No relationship appears to exist between the degree of DNA hybridization and the classes of isoleucine biosynthesis. Although the precise intermediates on the pyruvate pathway are unknown, the origin of the carbon skeleton of isoleucine synthesized by thi...Continue Reading

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Citations

Aug 21, 1987·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R H DunstanW Greenaway
Nov 16, 2010·Bioinformatics·Darong LaiChristine Nardini
Oct 31, 2009·Microbiology·Bing WuYinjie J Tang
Sep 25, 2012·Applied and Environmental Microbiology·Ambroise LambertMathieu Picardeau
Dec 29, 1998·Journal of Bacteriology·D M HowellR H White
May 13, 1999·Journal of Bacteriology·M HochuliT Szyperski
Jan 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Nobuko SugimotoRandolph Beaudry
Jul 1, 1988·Journal of Bacteriology·T NesbakkenJ Ormerod
Mar 16, 2013·Chemical Reviews·Christine A Rabinovitch-DeereShota Atsumi

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