PMID: 6399820Jan 1, 1984Paper

Multiple pathways for regenerating ribonuclease A

Advances in Biophysics
H A ScheragaT OOI

Abstract

This paper is concerned with the pathways for the regeneration of RNase A from the reduced protein by a mixture of GSSG and GSH. Experimental work on the regeneration has led to the identification of several different pathways, depending on the concentrations of GSH and GSSG, and an energetic analysis has provided information about the stabilities of the various intermediates. The equilibrium and kinetic data for the regeneration process have led to two models of protein-folding pathways. The intermediates in the regeneration process were trapped without chemical modification, and were fractionated on a carboxymethyl-cellulose column. The regeneration pathway(s) could be represented in terms of two simple reactions (Eqs. (1) and (2)). The system rapidly reaches a pre-equilibrium among the intermediates prior to the rate-limiting steps, and the concentrations of the intermediates (and hence the equilibrium constants among them) were determined. The regeneration process was also re-started from several of the isolated intermediates, and led to the predicted distribution of intermediates in the pre-equilibrium. Kinetic data, obtained by following the time dependence of the regain of enzymatic activity, together with the distributi...Continue Reading

Citations

Jan 1, 1993·Protein Science : a Publication of the Protein Society·C J Benham, M S Jafri
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H A ScheragaP W Mui
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T E Creighton
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D M Rothwarf, H A Scheraga
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J H LaityH A Scheraga
Apr 7, 2000·Protein Science : a Publication of the Protein Society·F VinciG Marino
May 7, 2011·Annual Review of Biophysics·Harold A Scheraga
Apr 9, 2015·Quarterly Reviews of Biophysics·Harold A Scheraga
Mar 16, 1993·Biochemistry·D M Rothwarf, H A Scheraga
Jul 1, 2004·The Journal of Biological Chemistry·Joan L ArolasSalvador Ventura
Sep 18, 2007·Biopolymers·Garland R MarshallDaniel J Kuster
Nov 17, 2007·Biopolymers·Harold A Scheraga
Jan 1, 1985·Annals of the New York Academy of Sciences·H A Scheraga
Apr 21, 1995·The Journal of Biological Chemistry·J Y ChangP H Lai
Jan 1, 1987·Progress in Biophysics and Molecular Biology·R Jaenicke
Dec 18, 1987·Biochimica Et Biophysica Acta·W Liu, C L Tsou
Jun 5, 2003·Biochemistry·Silvia SalamancaJui-Yoa Chang

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