Multiple pathways of Fas-induced apoptosis in primary culture of hepatocytes

Biochemical and Biophysical Research Communications
N RouquetV Joulin

Abstract

Fas (Apo1/CD95) is a member of the tumour necrosis factor/nerve growth factor receptor superfamily and mediates apoptosis in various cell types (for review sec [1]). Although this apoptotic activity has been clearly related to homeostasis in the immune system and pathological situations in non-lymphoid organs, the Fas signaling pathway remains mostly elusive. We and others previously showed that Fas-induced apoptosis of primary culture hepatocytes requires either an inhibitor of translation or a protein kinase inhibitor, suggesting that two distinct pathways of Fas signaling exist in hepatocytes. We report here that activation of ICE-like and CPP32-like cysteine proteases are required for Fas-mediated apoptosis, but that these pathways involve different subclasses of serine proteases and are selectively modulated by inhibitors of protein tyrosine kinases. These results confirm that distinct pathways can lead to Fas-induced apoptosis in hepatocytes. Further understanding of these pathways could facilitate the rational design of anti-apoptotic drugs in liver diseases associated with massive Fas-mediated hepatocyte apoptosis, including fulminant hepatitis.

Citations

Mar 17, 1999·The Journal of Experimental Medicine·A HakemJ M Penninger
Jan 26, 2011·Toxicology and Applied Pharmacology·Gong FengYansheng Bai
Oct 17, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mathieu VinkenTamara Vanhaecke
Dec 4, 2015·Toxicology and Applied Pharmacology·Michaël MaesHartmut Jaeschke
May 17, 2007·International Journal of Experimental Pathology·C T McCulloughD J Harrison
Nov 19, 2004·Biochemical Pharmacology·M J Gómez-LechónJ Mir
Aug 11, 2000·Seminars in Cancer Biology·S Kanzler, P R Galle
Jan 24, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shah JahanNadeem Afzal
Sep 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S F SchlosserJ L Boyer
Feb 3, 2004·Journal of Cellular Physiology·Nikolaus BresgenP M Eckl
Jun 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R A JonesG E Kass
Aug 8, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W GongA M Gressner
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S JiangJ H Park
Dec 5, 2009·Cellular and Molecular Life Sciences : CMLS·Mathieu VinkenVera Rogiers
Sep 10, 2013·Archives of Toxicology·Mathieu VinkenVera Rogiers
May 12, 2001·Critical Reviews in Clinical Laboratory Sciences·M G Neuman
Mar 2, 2011·Molecular and Cellular Biochemistry·Gong FengYansheng Bai
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Luca MarucciGene LeSage
Apr 15, 2000·The Journal of Biological Chemistry·E HatanoD A Brenner
May 21, 2009·Alternatives to Laboratory Animals : ATLA·Mathieu VinkenVera Rogiers
Dec 18, 1998·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·G FeldmannA Bringuier

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