Multiple peaks induced by domain-specific binding of fibrinogen in anion-exchange high-performance liquid chromatography

Journal of Chromatography. a
Y LiuJ R Shainoff

Abstract

A domain binding model was developed for explaining the multiple peak chromatograms obtained in the high-performance liquid chromatography of pure fibrinogen on a DEAE polymethacrylate column using different gradients of ammonium chloride. The different peaks for fibrinogen result from the binding of either the D or E domain of fibrinogen to the packing material. This was confirmed by comparing the retention times of the chromatograms for fibrinogen, fragment D1 and fragment E. Native and denatured forms of fibrinogen are proposed to be important to fibrinogen's interaction with the column, hiding or exposing the E domain, respectively. Different gradient speeds resolve a different number of peaks for fibrinogen, with slow gradients yielding essentially one peak and fast gradients 10 or more peaks. Temperature studies were done to confirm the model. Different commercial sources of fibrinogen showed different proportions of native and denatured/degraded forms.

References

May 27, 1977·Biochimica Et Biophysica Acta·N SemeraroM Verstraete
Mar 12, 1992·Biochimica Et Biophysica Acta·I Azpiazu, D Chapman
Jan 19, 1990·Journal of Chromatography·S W Lin, B L Karger
Jan 1, 1986·Critical Reviews in Oncology/hematology·A Z Budzynski
Jan 1, 1988·Critical Reviews in Clinical Laboratory Sciences·J A Shafer, D L Higgins
Dec 20, 1985·Science·J W WeiselC Cohen
Jun 27, 1983·Annals of the New York Academy of Sciences·H P Erickson, W E Fowler
Jun 27, 1983·Annals of the New York Academy of Sciences·M W Mosesson
Aug 25, 1982·Journal of Molecular Biology·P L Privalov, L V Medved
Oct 13, 1981·Biochemistry·C Wolfenstein-Todel, M W Mosesson
Dec 1, 1981·Scientific American·R F Doolittle
Aug 1, 1993·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·D R EllisR F Ebert
May 19, 1993·Journal of Chromatography·J P McConnell, D J Anderson

❮ Previous
Next ❯

Citations

Apr 20, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·F FerriM Rocco
Nov 18, 2008·Journal of Chromatography. a·Colin F Poole, Salwa K Poole

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.