Multiple Phosphatases Regulate Carbon Source-Dependent Germination and Primary Metabolism in Aspergillus nidulans

G3 : Genes - Genomes - Genetics
Leandro José de AssisNeil Andrew Brown

Abstract

Aspergillus nidulans is an important mold and a model system for the study of fungal cell biology. In addition, invasive A. nidulans pulmonary infections are common in humans with chronic granulomatous disease. The morphological and biochemical transition from dormant conidia into active, growing, filamentous hyphae requires the coordination of numerous biosynthetic, developmental, and metabolic processes. The present study exhibited the diversity of roles performed by seven phosphatases in regulating cell cycle, development, and metabolism in response to glucose and alternative carbon sources. The identified phosphatases highlighted the importance of several signaling pathways regulating filamentous growth, the action of the pyruvate dehydrogenase complex as a metabolic switch controlling carbon usage, and the identification of the key function performed by the α-ketoglutarate dehydrogenase during germination. These novel insights into the fundamental roles of numerous phosphatases in germination and carbon sensing have provided new avenues of research into the identification of inhibitors of fungal germination, with implications for the food, feed, and pharmaceutical industries.

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Citations

Jul 6, 2016·Fungal Genetics and Biology : FG & B·Michaela NovodvorskaDavid B Archer
May 11, 2017·Journal of Applied Microbiology·F J J SegersJ Dijksterhuis
May 26, 2018·G3 : Genes - Genomes - Genetics·Laure Nicolas Annick RiesGustavo H Goldman
May 7, 2015·G3 : Genes - Genomes - Genetics·Lizziane K WinkelströterGustavo H Goldman
Jul 31, 2019·Scientific Reports·Aroa Rodriguez-Iglesias, Monika Schmoll
Jun 16, 2021·The ISME Journal·Flávia P FrancoMarcio C Silva-Filho
Jul 28, 2021·MBio·Laure Nicolas Annick RiesGustavo H Goldman

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Methods Mentioned

BETA
dissection
protein assay
Assay

Software Mentioned

Prism
FunCat
BLASTp
Mev
MIPS
FetGOat
GraphPad
Venn

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