Multiple polymorphic sites at the ITS and ATAN loci in cultured isolates of Perkinsus marinus

The Journal of Eukaryotic Microbiology
Gwynne D BrownK S Reece

Abstract

Sequence analysis of genomic DNA from the protozoan parasite Perkinsus marinus at two loci revealed genetic polymorphisms within and among different cultured isolates. Genomic DNA from 12 Perkinsus marinus isolates was amplified at the internal transcribed spacer region and at an anonymous locus previously identified to contain polymorphisms by restriction fragment length polymorphism analysis. Fourteen polymorphic nucleotide positions were identified at the internal transcribed spacer region; eight in internal transcribed spacer 1 and six in internal transcribed spacer 2. Thirteen polymorphic nucleotide sites were identified within the anonymous locus. In some instances, more than three different sequences were observed at both the internal transcribed spacer region and at the anonymous locus from a single clonal isolate, suggesting the possibility of recombination in cultured cells and/or strand jumping during the polymerase chain reaction. Intra-isolate sequence variation (3.46% for the anonymous locus and 3.08% for internal transcribed spacer 1) was in several cases as high as inter-isolate sequence variation, even in one isolate where recombination was not evident. High intra- and inter-isolate variation detected at both l...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1991·The Quarterly Review of Biology·D M Hillis, M T Dixon
Aug 9, 1988·Biochemistry·K R Tindall, T A Kunkel
Jun 1, 1984·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·R N Nazar
Jun 7, 1993·Journal of Theoretical Biology·D J Colgan
Feb 1, 1996·Applied and Environmental Microbiology·M CarrawayG Widmer
Apr 1, 1996·International Journal for Parasitology·S Payne, J Ellis
Apr 6, 2000·The Journal of Eukaryotic Microbiology·D BushekK S Reece
Aug 3, 2002·Diseases of Aquatic Organisms·Christopher F DunganKimberly S Reece
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R. CronnJ. F. Wendel

❮ Previous
Next ❯

Citations

Nov 6, 2004·Applied and Environmental Microbiology·Corinne AudemardEugene M Burreson
Jun 26, 2008·The Journal of Parasitology·Richard W GerholdJohn R Fischer
Nov 14, 2014·Diseases of Aquatic Organisms·J M ValenciaJ A Castro
Nov 21, 2009·European Journal of Protistology·Kristina M HamiltonPaul W Shaw
Jan 6, 2009·The Journal of Eukaryotic Microbiology·Karl E Miletti-González, Michael J Leibowitz
Aug 14, 2013·Molecular Ecology Resources·Sabine Giessler, Justyna Wolinska
Apr 1, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Peter C ThompsonMatthew P Hare
Jun 2, 2005·The Journal of Eukaryotic Microbiology·Eugene M BurresonChristopher F Dungan

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY299656-AY299683
AY527398
AF150990

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

GenePop
PAUP
CLUSTALW
UPGMA
BLAST ( Basic Local Alignment Search Tool ) Search
MacVector
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.