PMID: 9524241Apr 3, 1998Paper

Multiple promoters are responsible for transcription of the glpEGR operon of Escherichia coli K-12

Biochimica Et Biophysica Acta
B Yang, Timothy J Larson

Abstract

The transcriptional organization of the glpEGR genes of Escherichia coli was studied. Besides a promoter located upstream of the glpE start codon, three internal glpGR promoters were identified that express glpG and/or glpR (glp repressor). One promoter was located just upstream of the glpG start codon and two others (separated by several hundred base pairs) were located within glpG upstream of the glpR start codon. The transcriptional start points of these promoters were identified by primer extension analysis. The strengths of the individual promoters were compared by analysis of their expression when fused to a pormoter-probe vector. Analysis of the transcriptional expression of the glpEGR sequence with different combinations of the glpEGR promoters revealed no internal transcriptional terminators within the entire operon. Thus, the glpEGR genes are co-transcribed and form a single complex operon. The presence of multiple promoters may provide for differential expression of glpE, glpG and glpR. Potential regulation of the operon promoters by GlpR, catabolite repression, anaerobiosis or by FIS was studied. The glpE promoter was apparently controlled by the cAMP-CRP complex, but none of the promoters was responsive to specific...Continue Reading

References

Feb 14, 1968·Journal of Molecular Biology·N R CozzarelliE C Lin
Oct 1, 1996·Molecular Microbiology·G González-GilR Kahmann
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H E HuberT A Bickle

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Citations

Aug 19, 2007·Journal of Bacteriology·Chris Guillot, Charles P Moran
May 18, 2010·Journal of Bacteriology·Marija TauschekRoy M Robins-Browne
Dec 17, 2004·Journal of Bacteriology·Karina B Xavier, Bonnie L Bassler
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Ross E DalbeyJan Maarten van Dijl
Sep 24, 2004·Research in Microbiology·M Joanne LemieuxDa-Neng Wang
Dec 23, 2015·Molecular Microbiology·A Vimala, R Harinarayanan
Mar 23, 2013·Biochimica Et Biophysica Acta·Philip Rather

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