Multiple proteases to localize oxidation sites

PloS One
Liqing Gu, Renã A S Robinson

Abstract

Proteins present in cellular environments with high levels of reactive oxygen and nitrogen species and/or low levels of antioxidants are highly susceptible to oxidative post-translational modification (PTM). Irreversible oxidative PTMs can generate a complex distribution of modified protein molecules, recently termed as proteoforms. Using ubiquitin as a model system, we mapped oxidative modification sites using trypsin, Lys-C, and Glu-C peptides. Several M+16 Da proteoforms were detected as well as proteoforms that include other previously unidentified oxidative modifications. This work highlights the use of multiple protease digestions to give insights to the complexity of oxidative modifications possible in bottom-up analyses.

References

Aug 1, 1992·Archives of Biochemistry and Biophysics·Z MaskosW H Koppenol
May 1, 1997·Chemical Research in Toxicology·E R Stadtman, B S Berlett
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
Apr 13, 2000·Journal of Cellular Biochemistry. Supplement·A CiechanoverA L Schwartz
Nov 23, 2000·Nature·T Finkel, N J Holbrook
May 18, 2001·Postȩpy higieny i medycyny doświadczalnej·A AugustyniakE Skrzydlewska
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Siu Kwan SzeFred W McLafferty
Feb 28, 2003·Analytical Biochemistry·Joshua S SharpRobert L Hettich
Sep 23, 2003·Protein Science : a Publication of the Protein Society·Ying GeFred W McLafferty
Jun 14, 2005·Biochemical and Biophysical Research Communications·Ray Bakhtiar, Ziqiang Guan
Oct 15, 2005·Phytochemical Analysis : PCA·Susana I PereiraArtur M S Silva
Jan 13, 2006·Methods in Enzymology·J Mitchell Wells, Scott A McLuckey
Jan 13, 2006·Methods in Enzymology·Robert J ChalkleyMichael A Baldwin
Nov 23, 2006·Biochimica Et Biophysica Acta·Leann M MikeshDonald F Hunt
Mar 28, 2008·Journal of Proteome Research·Nicolas J HauserFranco Basile
Mar 19, 2009·Mass Spectrometry Reviews·Jennifer S Brodbelt, Jeffrey J Wilson
Feb 2, 2010·Journal of Proteome Research·Danielle L SwaneyJoshua J Coon
Feb 4, 2010·Journal of Proteome Research·Ashraf G Madian, Fred E Regnier
Apr 27, 2010·Journal of the American Society for Mass Spectrometry·Natacha LouretteLjiljana Pasa-Tolić
Jun 5, 2010·Journal of Proteome Research·Ashraf G Madian, Fred E Regnier
May 3, 2011·Free Radical Biology & Medicine·Fu Shang, Allen Taylor
May 24, 2011·Journal of Proteomics·Ian M MøllerR S P Rao
Nov 23, 2011·Molecular & Cellular Proteomics : MCP·Ashraf G MadianFred E Regnier
Dec 23, 2011·Current Pharmaceutical Design·Marc Kastle, Tilman Grune
Dec 31, 2011·The Journal of Biological Chemistry·Liwen WangVera Y Moiseenkova-Bell
Jan 10, 2012·Rapid Communications in Mass Spectrometry : RCM·Kevin M DownardSatoko Akashi
Dec 6, 2012·The Journal of Physical Chemistry. B·Huilin ShiRenã A S Robinson
Feb 28, 2013·Nature Methods·Lloyd M SmithUNKNOWN Consortium for Top Down Proteomics
Jul 9, 2013·Mass Spectrometry Reviews·Maria FedorovaRalf Hoffmann
Sep 12, 2013·Molecular & Cellular Proteomics : MCP·Adam D CathermanNeil L Kelleher

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Methods Mentioned

BETA
electrophoresis
footprinting
reversed-phase chromatography
amidation

Software Mentioned

ProteinProspector
Proteome Discoverer
Sequest
Xtract
Xcalibur

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