Abstract
Nucleocytosolic and secreted proteins are commonly glycosylated. However, reports of glycosylated mitochondrial proteins are rare. Using lectin chromatography on bovine heart, we detected low-abundance glycoforms of nuclear-encoded proteins with well-established mitochondrial function: pyruvate dehydrogenase E1α, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, ADP/ATP translocase, ATP synthase d and oligomycin sensitivity-conferring protein. Notably, the latter two have been previously detected at the plasma membrane. Our findings indicate that glycosylation of classic mitochondrial proteins may be more common than previously appreciated. We discuss the implication that glycosylation could represent an unexplored mechanism for regulating these proteins' functions within mitochondria or at extra-mitochondrial locations.
References
Nov 27, 1979·Biochemistry·B P PetersM Flashner
Jan 1, 1989·The International Journal of Biochemistry·C LevratP Louisot
Jul 25, 1998·The Journal of Biological Chemistry·N C ChandraR G Spiro
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·T L MoserS V Pizzo
Jan 23, 2003·Journal of Cell Science·Dona C LoveJarema Kochran
May 29, 2004·The Journal of Biological Chemistry·Chen ChenPeter L Pedersen
Jun 3, 2004·Proteomics·Nikolaj BlomSøren Brunak
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Mar 30, 2005·Biochimica Et Biophysica Acta·Richard D MendelsohnMaria A Kukuruzinska
Jan 18, 2006·Analytical Chemistry·Animesh NandiYingming Zhao
Sep 9, 2006·Cell·Kazuaki Ohtsubo, Jamey D Marth
Nov 23, 2006·Mitochondrion·Sarah K Yonally, Roderick A Capaldi
May 5, 2007·Biochimica Et Biophysica Acta·Anne M DistlerCharles L Hoppel
Jul 24, 2007·Molecular Immunology·Pierre VantouroutEric Champagne
Aug 30, 2007·Molecular Psychiatry·C SchmidtM Schachner
Jul 17, 2008·Archives of Physiology and Biochemistry·Janos KernerCharles L Hoppel
Aug 8, 2008·Journal of the American Chemical Society·Peter M ClarkLinda C Hsieh-Wilson
Oct 11, 2008·Nature Reviews. Immunology·Jamey D Marth, Prabhjit K Grewal
Nov 14, 2008·The Journal of Biological Chemistry·Yong HuWolfgang H Dillmann
Sep 17, 2009·Molecular Systems Biology·Li A KungHeng Zhu
Mar 17, 2010·PLoS Biology·Ohad YogevOphry Pines
Mar 23, 2010·Nature Chemical Biology·Chin Fen TeoGeert-Jan Boons
Jan 22, 2011·Folia Microbiologica·M HričínováS Ondrašovičová
Apr 7, 2011·BMC Bioinformatics·Jinlian WangZhang-Zhi Hu
Sep 13, 2011·Proteomics·Reut Ben-MenachemOphry Pines
Citations
Sep 10, 2013·Biochemistry. Biokhimii︠a︡·G Ya Wiederschain
Nov 16, 2012·PloS One·Amanda R Burnham-MarusichPatricia M Berninsone
Oct 22, 2013·International Journal of Molecular Sciences·Xiaojin WangLin Liu
May 3, 2014·International Journal of Molecular Sciences·Manuela AntonielGiovanna Lippe
Aug 16, 2016·Pesticide Biochemistry and Physiology·Jixing XiaYoujun Zhang
Nov 15, 2012·The EMBO Journal·Werner J H KoopmanPeter H G M Willems
Aug 27, 2014·Biochimica Et Biophysica Acta·Shyam Kankotia, Peter W Stacpoole
Nov 7, 2013·Journal of Proteome Research·Gaigai WangJiahao Sha
Dec 13, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiao-Tong MaYu-Kui Zhang
Apr 30, 2016·Molecular & Cellular Proteomics : MCP·Thibaut LégerJean-Michel Camadro
Jul 29, 2016·Molecular & Cellular Proteomics : MCP·Alessandra RavidàSandra M O'Neill
Oct 24, 2017·Journal of the National Cancer Institute·Peter W Stacpoole
Sep 15, 2018·Diabetes·Chris ShannonRalph A DeFronzo
Oct 7, 2018·British Journal of Pharmacology·Valentina GiorgioPaolo Bernardi
May 24, 2019·Analytical and Bioanalytical Chemistry·Xian ZhangTianying Guo
Jun 13, 2017·Frontiers in Medicine·Timothy GallCornelius F Boerkoel
Apr 10, 2014·The Journal of Biological Chemistry·Ee Phie TanChad Slawson
Jan 20, 2017·The Journal of Biological Chemistry·Juliana L SacomanPatricia M Berninsone
Jun 23, 2018·Frontiers in Neuroscience·Paula A Q Videira, Margarida Castro-Caldas
Dec 24, 2019·Cell Metabolism·Hee Chan YooJung Min Han
Mar 18, 2021·Environmental Microbiology·Can ZhangXili Liu
Aug 14, 2021·Proceedings of the National Academy of Sciences of the United States of America·Hongjie GuoStephen M Beverley