PMID: 9171106Jun 15, 1997Paper

Multiple regions of p45 NF-E2 are required for beta-globin gene expression in erythroid cells

Nucleic Acids Research
T L Bean, P A Ney

Abstract

Regulated expression of genes in the beta-globin cluster depends upon sequences located between 5 and 20 kb upstream of the epsilon gene, known as the locus control region (LCR). beta-Globin expression in murine erythroleukemia (MEL) cells depends on NF-E2, a transcription factor which binds to enhancer sequences in the LCR. To gain insight into the mechanism of globin gene activation by NF-E2, an NF-E2 null MEL cell line was used to map regions of NF-E2 required for beta-globin expression. Within the transactivation domain, two discrete proline-rich regions were required for rescue of beta-globin expression. The first was located at the N-terminus of NF-E2, while the second was located N-terminal of the cap 'n collar (CNC) domain. Other proline-rich sequences were dispensable, indicating that proline content per se does not determine NF-E2 activity. Mutations within the conserved CNC domain markedly diminished rescue of beta-globin expression. This domain was required, in addition to the basic leucine zipper domain, for DNA binding activity. The requirement for discrete proline-rich sequences within the transactivation domain suggests that globin gene expression in MEL cells depends on specific interactions between NF-E2 and d...Continue Reading

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Citations

Feb 27, 2001·Brain Research. Molecular Brain Research·M DoyuK Kato
Oct 31, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y KatohM Yamamoto
Mar 31, 2010·Molecular and Cellular Biology·Mariko TakayamaMasayuki Yamamoto
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Aug 3, 2021·The Journal of Biological Chemistry·Kazuhiko IgarashiMitsuyo Matsumoto

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