PMID: 8971025Dec 1, 1996Paper

Multiple regulatory effects of varicella-zoster virus (VZV) gL on trafficking patterns and fusogenic properties of VZV gH

Journal of Virology
Karen M Duus, C Grose

Abstract

Varicella-zoster virus (VZV) is an extremely cell-associated alphaherpesvirus; VZV infection is spread almost exclusively via cell membrane fusion. The envelope glycoprotein H (gH) is highly conserved among the herpesviruses. A virus-encoded chaperone, glycoprotein L (gL), associates with gH, and the gH:gL complex is required for gH maturation and membrane expression. We recently demonstrated that in the VZV system, the gH:gL complex facilitated cell membrane fusion and extensive polykaryon formation in transfected cells (K. M. Duus, C. Hatfield, and C. Grose, Virology 210:429-440, 1995). To further define the functions of the unusual VZV gL chaperone protein, we have performed a series of mutagenesis experiments with both gH and gL and analyzed the mutants by laser scanning confocal microscopy in a transfection-based fusion assay. We established the fact that immature gH exited the endoplasmic reticulum (ER) when coexpressed with either gE or gI and appeared on the cell surface in a patch pattern. A similar effect was observed on the cell surface with gH with a cytoplasmic tail mutagenized to closely resemble the vaccinia virus hemagglutinin cytoplasmic tail. Site-directed mutagenesis of the five gL cysteine residues demonstra...Continue Reading

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Citations

Jun 23, 2006·Reviews in Medical Virology·Mark Quinlivan, Judith Breuer
Jun 24, 2003·Reviews in Medical Virology·Nancy L Cole, Charles Grose
Jun 20, 2013·BioMed Research International·Longfeng JiangMingli Wang
Nov 14, 2014·Journal of Virology·Sebastian W BöhmWalter Fuchs
Jan 29, 2010·Journal of Virology·Tomohiko SadaokaYasuko Mori
May 3, 2008·The Journal of Infectious Diseases·Johnathan StorlieCharles Grose
Sep 16, 2006·Journal of Virology·Simon Metz PedersenPer Höllsberg
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Susan E VleckAnn M Arvin

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