PMID: 2118511Sep 1, 1990Paper

Multiple regulatory sites in the Bacillus subtilis citB promoter region

Journal of Bacteriology
A FouetAbraham L Sonenshein

Abstract

The aconitase (citB) gene of Bacillus subtilis is repressed during growth in a medium that contains a rapidly metabolizable carbon source and a source of 2-ketoglutarate. It is derepressed when either of these nutrient sources becomes limiting. Repression by rapidly metabolizable carbon sources was shown previously to depend at least in part on a DNA sequence located 67 to 84 base pairs upstream of the start point of citB transcription. In the present work, this region and surrounding DNA were mutagenized to identify more precisely the target for carbon catabolite repression. Mutations in a symmetric sequence located between positions -73 and -59 led to constitutive transcription from the citB promoter in media that normally provoke catabolite repression. By gel mobility shift assays, it was shown that at least one protein in extracts of B. subtilis binds to the symmetric sequence and that DNA of constitutive mutants binds to this protein much less effectively. A second sequence located near position -45 was also implicated in this regulation. A second form of regulation of citB was also investigated. This gene is known to be derepressed when cells are induced to sporulate by exhaustion of a nutrient broth medium or limitation ...Continue Reading

References

Oct 4, 1979·Biochimica Et Biophysica Acta·J M LopezE Freese
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1990·Genes & Development·N MandalH Echols
Oct 1, 1985·Journal of Bacteriology·M S RosenkrantzA L Sonenshein
Jul 1, 1987·Journal of Bacteriology·D W DingmanA L Sonenshein
Apr 8, 1988·Science·R Schleif
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke

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Citations

Jan 19, 1999·Annual Review of Microbiology·M M Nakano, P Zuber
Apr 1, 1995·Current Microbiology·E A Lottering, U N Streips
Feb 20, 2003·Journal of Bacteriology·Hyun-Jin KimMark Strauch
Jun 27, 1998·Journal of Bacteriology·M M NakanoA L Sonenshein
Feb 1, 1997·Journal of Bacteriology·D G CvitkovitchA S Bleiweis
Sep 1, 1991·Research in Microbiology·I SmithN Gaur
Aug 1, 1994·Journal of Bacteriology·S Jin, A L Sonenshein
Aug 1, 1994·Journal of Bacteriology·S Jin, A L Sonenshein

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