PMID: 7535921Mar 11, 1995Paper

Multiple RNA binding domains (RBDs) just don't add up

Nucleic Acids Research
Y ShamooK R Williams

Abstract

One of the most common motifs for binding RNA in eukaryotes is the RNA binding domain (RBD) or RNA Recognition Motif (RRM). One of the more intriguing aspects of these proteins is their modular nature. Proteins have been found containing from one to four RRMs. In most instances, these domains have some basal level of non-sequence specific RNA binding affinity. In addition, many also have a higher affinity for a specific structure or sequence of RNA. In the cases of heterogenous nuclear ribonucleoprotein A1 (hnRNP A1), yeast poly-A binding protein and splicing factor U2AF65, the individual free energy of binding of the RBDs for RNA are not strictly additive. By invoking a model in which the amino acids connecting adjoining RBDs are considered to be flexible linkers with an interresidue spacing of about 3.5 A, it is possible to predict the apparent association constants for at least some multi-RBD proteins to single-stranded RNA. We have surveyed the literature and found that individual RBDs are separated by 'linker' sequences of highly variable length. These linkers provide a critical determinant of binding affinity and may modulate cis versus trans binding. A clearer understanding of multi-RBD binding is essential to critically...Continue Reading

References

Jun 1, 1991·Trends in Biochemical Sciences·D J KenanJ D Keene
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A MayedaM B Roth
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D W HoffmanJ D Keene
Mar 1, 1972·Immunochemistry·D M Crothers, H Metzger

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Citations

Apr 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Graumann, M A Marahiel
May 31, 2001·Journal of Molecular Graphics & Modelling·A K DunkerZ Obradovic
May 3, 2007·Nature Reviews. Molecular Cell Biology·Bradley M LundeGabriele Varani
Mar 1, 1997·Nature Structural Biology·Y ShamooT A Steitz
Aug 17, 2010·Nature Structural & Molecular Biology·Cyprian D CukierAndres Ramos
Jan 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elena Khazina, Oliver Weichenrieder
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Guang-Yao LiC Anne Koch
Aug 15, 2002·Genome Research·Juri RappsilberMatthias Mann
May 10, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·E Allen SickmierClara L Kielkopf
Aug 26, 2009·Molecular and Cellular Biology·Pär LundkvistLars Wieslander
Jun 10, 2000·Molecular and Cellular Biology·E LalliP Sassone-Corsi
Aug 19, 2005·Molecular and Cellular Biology·Matthias Soller, Kalpana White
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·G Varani, K Nagai
May 16, 2001·Biochemical and Biophysical Research Communications·P P LauL Chan
Jun 14, 2014·Progress in Nuclear Magnetic Resonance Spectroscopy·Christoph GöblMichael Sattler
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S M CrowderT Alber
Jul 30, 2005·FEBS Letters·Berenike KleinhenzSusanne Illenberger
Nov 17, 2004·The Journal of Cell Biology·Akwasi AnyanfulShoichiro Ono
Apr 21, 2012·Current Opinion in Structural Biology·Cameron D Mackereth, Michael Sattler
Jul 31, 2010·Journal of Biomedicine & Biotechnology·Paulo Navarro-CostaJoão Gonçalves
Nov 21, 2007·Journal of Molecular Biology·Pritilekha DekaGabriele Varani
Nov 3, 2006·Biopolymers·Micah J McCauley, Mark C Williams

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