Multiple RNA processing defects and impaired chloroplast function in plants deficient in the organellar protein-only RNase P enzyme

PloS One
Wenbin ZhouRalph Bock

Abstract

Transfer RNA (tRNA) precursors undergo endoribonucleolytic processing of their 5' and 3' ends. 5' cleavage of the precursor transcript is performed by ribonuclease P (RNase P). While in most organisms RNase P is a ribonucleoprotein that harbors a catalytically active RNA component, human mitochondria and the chloroplasts (plastids) and mitochondria of seed plants possess protein-only RNase P enzymes (PRORPs). The plant organellar PRORP (PRORP1) has been characterized to some extent in vitro and by transient gene silencing, but the molecular, phenotypic and physiological consequences of its down-regulation in stable transgenic plants have not been assessed. Here we have addressed the function of the dually targeted organellar PRORP enzyme in vivo by generating stably transformed Arabidopsis plants in which expression of the PRORP1 gene was suppressed by RNA interference (RNAi). PRORP1 knock-down lines show defects in photosynthesis, while mitochondrial respiration is not appreciably affected. In both plastids and mitochondria, the effects of PRORP1 knock-down on the processing of individual tRNA species are highly variable. The drastic reduction in the levels of mature plastid tRNA-Phe(GAA) and tRNA-Arg(ACG) suggests that these ...Continue Reading

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Feb 21, 2016·Nucleic Acids Research·Nadia BrillanteRoland K Hartmann
Dec 30, 2015·The Plant Journal : for Cell and Molecular Biology·Birgit Stoll, Stefan Binder
Aug 14, 2015·BMC Plant Biology·Alice Louise BurgessIain Robert Searle
Jun 30, 2018·RNA·Jinjie ShangYing Huang

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Methods Mentioned

BETA
transgenic
PCR
light microscopy
electrophoresis
protein assay
transmission electron microscopy
RNAseq
Illumina sequencing

Software Mentioned

R
cell
Samtools
BWA
intersectBed
FastQC
BEDtools
BEDtools package

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