Multiple roles for the MyoD basic region in transmission of transcriptional activation signals and interaction with MEF2.

Molecular and Cellular Biology
B L BlackE N Olson

Abstract

Establishment of skeletal muscle lineages is controlled by the MyoD family of basic helix-loop-helix (bHLH) transcription factors. The ability of these factors to initiate myogenesis is dependent on two conserved amino acid residues, alanine and threonine, in the basic domains of these factors. It has been postulated that these two residues may be responsible for the initiation of myogenesis via interaction with an essential myogenic cofactor. The myogenic bHLH proteins cooperatively activate transcription and myogenesis through protein-protein interactions with members of the myocyte enhancer factor 2 (MEF2) family of MADS domain transcription factors. MyoD-E12 heterodimers interact with MEF2 proteins to synergistically activate myogenesis, while homodimers of E12, which lack the conserved alanine and threonine residues in the basic domain, do not interact with MEF2. We have examined whether the myogenic alanine and threonine in the MyoD basic region are required for interaction with MEF2. Here, we show that substitution of the MyoD basic domain with that of E12 does not prevent interaction with MEF2. Instead, the inability of alanine-threonine mutants of MyoD to initiate myogenesis is due to a failure to transmit transcriptio...Continue Reading

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T J BrennanE N Olson
Aug 1, 1991·Genes & Development·H WeintraubS J Tapscott
Sep 1, 1990·Genes & Development·E N Olson
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H WeintraubA Lassar
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D LeiferS A Lipton
Mar 15, 1995·Genes & Development·B A BourH T Nguyen
Apr 1, 1995·European Journal of Biochemistry·P Shore, A D Sharrocks
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·H T NguyenN A Spoerel
Mar 1, 1994·Molecular and Cellular Biology·J F MartinE N Olson
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·B LillyE N Olson
Apr 1, 1993·Molecular and Cellular Biology·J C McDermottB Nadal-Ginard
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J F MartinE N Olson
Jul 1, 1996·Molecular and Cellular Biology·J D MolkentinE N Olson

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Citations

Apr 28, 2009·Advanced Drug Delivery Reviews·Jelena Mann, Derek A Mann
Mar 6, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Peng XuYongli Xu
Aug 16, 2005·Seminars in Cell & Developmental Biology·Charlotte A Berkes, Stephen J Tapscott
Aug 10, 2005·Seminars in Cell & Developmental Biology·Dan LuoThomas A Rando
May 23, 2014·Biochimica Et Biophysica Acta·M GanassiS Molinari
Feb 16, 2010·Chemico-biological Interactions·Shelley CampPalmer Taylor
Jun 13, 2013·Current Opinion in Genetics & Development·Abraham P Fong, Stephen J Tapscott
Oct 15, 2008·Molecular Cell·Maria Divina E DeatoRobert Tjian
Jan 19, 1999·Annual Review of Cell and Developmental Biology·B L Black, E N Olson
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·S S Hook, A R Means
Jan 20, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Maja Z SalvaStephen D Hauschka
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·J LuE N Olson
Apr 1, 2011·European Journal of Cardiovascular Prevention and Rehabilitation : Official Journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology·Giuseppe MaiolinoGian Paolo Rossi
Feb 7, 2004·European Journal of Clinical Investigation·J FredrikssonM Orho-Melander
Feb 13, 2004·American Journal of Physiology. Cell Physiology·Lubna Al-KhaliliAnna Krook
Jun 23, 2004·Nature Genetics·Cristiano SimonePier Lorenzo Puri
Jul 1, 2004·Nature Genetics·Mark A Gillespie, Michael A Rudnicki
Sep 15, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hongbin JiangJoseph X DiMario
Dec 15, 2004·Animal Biotechnology·J Wyszyńska-Koko, J Kurył
May 2, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Philip MarshallRonald G Worton
May 2, 2008·Journal of Cellular Physiology·Uri David AkaviaDafna Benayahu
Mar 7, 2013·Genetic Testing and Molecular Biomarkers·Yuxiang DaiWei Wu
Dec 11, 2013·Tissue Engineering. Part B, Reviews·Serge OstrovidovAli Khademhosseini
Dec 5, 2018·The Journal of Physiology·Abdulrahman JamaHongmei Ren
Mar 26, 2010·The Biochemical Journal·Sheng Pin Hsiao, Shen Liang Chen
Jun 16, 2009·The Biochemical Journal·Sheng P HsiaoShen L Chen

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