Multiple roles of afadin in the ultrastructural morphogenesis of mouse hippocampal mossy fiber synapses

The Journal of Comparative Neurology
Kousyoku SaiAkira Mizoguchi

Abstract

A hippocampal mossy fiber synapse, which is implicated in learning and memory, has a complex structure in which mossy fiber boutons attach to the dendritic shaft by puncta adherentia junctions (PAJs) and wrap around a multiply-branched spine, forming synaptic junctions. Here, we electron microscopically analyzed the ultrastructure of this synapse in afadin-deficient mice. Transmission electron microscopy analysis revealed that typical PAJs with prominent symmetrical plasma membrane darkening undercoated with the thick filamentous cytoskeleton were observed in the control synapse, whereas in the afadin-deficient synapse, atypical PAJs with the symmetrical plasma membrane darkening, which was much less in thickness and darkness than those of the control typical PAJs, were observed. Immunoelectron microscopy analysis revealed that nectin-1, nectin-3, and N-cadherin were localized at the control typical PAJs, whereas nectin-1 and nectin-3 were localized at the afadin-deficient atypical PAJs to extents lower than those in the control synapse and N-cadherin was localized at their nonjunctional flanking regions. These results indicate that the atypical PAJs are formed by nectin-1 and nectin-3 independently of afadin and N-cadherin and...Continue Reading

References

Jun 28, 2000·Neuroscience·D A HenzeG Barrionuevo
Feb 6, 2002·The Journal of Cell Biology·Akira MizoguchiYoshimi Takai
Jun 26, 2004·Annual Review of Neuroscience·Thomas C Sudhof
Sep 29, 2004·Neuron·Robert C Malenka, Mark F Bear
Dec 22, 2004·Nature Reviews. Neuroscience·Silvio O Rizzoli, William J Betz
Jun 28, 2006·Pflügers Archiv : European journal of physiology·Josef BischofbergerPeter Jonas
Jul 6, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Audrey MercerAlex M Thomson
Sep 28, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Astrid RollenhagenJoachim H R Lübke
Nov 23, 2007·Neuron·Sang Jeong Kim, David J Linden
May 6, 2008·Nature Neuroscience·Paul YoungGuoping Feng
May 13, 2008·Neuroscience·M S SantosS M Voglmaier
Jun 14, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zhong XiePeter Penzes
Jul 3, 2008·Annual Review of Cell and Developmental Biology·Yoshimi TakaiYoshiyuki Rikitake
Jul 24, 2008·Nature Reviews. Molecular Cell Biology·Yoshimi TakaiHisakazu Ogita
Sep 2, 2011·The Journal of Biological Chemistry·Souichi KuritaYoshimi Takai
Jan 6, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gerard M J BeaudoinLouis F Reichardt
Jan 13, 2012·The Journal of Comparative Neurology·Shanting ZhaoMichael Frotscher
May 18, 2012·Molecular Biology of the Cell·Nariaki IwasawaIzumi Oinuma
Sep 6, 2012·The Journal of Biological Chemistry·Deepak P SrivastavaPeter Penzes
Jan 11, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Scott A WilkeAnirvan Ghosh
May 4, 2013·The Journal of Investigative Dermatology·Indrajyoti IndraSergey Troyanovsky
Jul 22, 2014·The Journal of Comparative Neurology·Maria Elizabeth BellKristen M Harris
Jul 15, 2015·Molecular and Cellular Neurosciences·Takeshi FujiwaraAkira Mizoguchi
Nov 29, 2015·The Journal of Physiology·Simon Chamberland, Katalin Tóth
Dec 22, 2015·Molecular and Cellular Neurosciences·Tomohiko MaruoMasahiro Mori

❮ Previous
Next ❯

Citations

Jul 12, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tomohiko MaruoYoshimi Takai
Jun 21, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Xiaoqi GengMasahiro Mori
Jul 10, 2021·Molecular and Cellular Neurosciences·Kiyohito MizutaniYoshimi Takai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.