Multiple roles of core protein linker in hepatitis B virus replication.

PLoS Pathogens
Kuancheng LiuJianming Hu

Abstract

Hepatitis B virus (HBV) core protein (HBc) contains an N-terminal domain (NTD, assembly domain) and a C-terminal domain (CTD), which are linked by a flexible linker region. HBc plays multiple essential roles in viral replication, including capsid assembly, packaging of the viral pregenomic RNA (pgRNA) into nucleocapsids, viral reverse transcription that converts pgRNA to the genomic DNA, and secretion of DNA-containing (complete) virions or genome-free (empty) virions. The HBc linker is generally assumed to act merely as a spacer between NTD and CTD but some results suggest that the linker may affect NTD assembly. To determine its role in viral replication, we have made a number of deletion and substitution mutants in the linker region, in either the presence or absence of CTD, and tested their abilities to support capsid assembly and viral replication in human cells. Our results indicate that the linker could indeed impede NTD assembly in the absence of CTD, which could be partially relieved by partial linker deletion. In contrast, when CTD was present, the linker deletions or substitutions did not affect capsid assembly. Deletion of the entire linker or its C-terminal part resulted in a partial defect in pgRNA packaging and s...Continue Reading

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Citations

Aug 4, 2019·Journal of Virology·Jianming HuAleem Siddiqui
Nov 18, 2018·Frontiers in Microbiology·Fan Yang
Sep 6, 2020·Cells·Bingfu Jiang, Eberhard Hildt
Jun 12, 2020·World Journal of Gastroenterology : WJG·Marçal YllFrancisco Rodríguez-Frías
Nov 6, 2020·Journal of Virology·Xupeng HongJianming Hu
May 7, 2020·Journal of Molecular Biology·Virgile RatHugues de Rocquigny
Aug 24, 2021·Frontiers in Cellular and Infection Microbiology·Michal Slawomir BarskiGoedele Noella Maertens

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Methods Mentioned

BETA
electrophoresis
transfection
PCR

Software Mentioned

BioLab software

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