Multiple roles of integrin-linked kinase in epidermal development, maturation and pigmentation revealed by molecular profiling.

PloS One
David JudahLina Dagnino

Abstract

Integrin-linked kinase (ILK) is an important scaffold protein that mediates a variety of cellular responses to integrin stimulation by extracellular matrix proteins. Mice with epidermis-restricted inactivation of the Ilk gene exhibit pleiotropic phenotypic defects, including impaired hair follicle morphogenesis, reduced epidermal adhesion to the basement membrane, compromised epidermal integrity, as well as wasting and failure to thrive leading to perinatal death. To better understand the underlying molecular mechanisms that cause such a broad range of alterations, we investigated the impact of Ilk gene inactivation on the epidermis transcriptome. Microarray analysis showed over 700 differentially regulated mRNAs encoding proteins involved in multiple aspects of epidermal function, including keratinocyte differentiation and barrier formation, inflammation, regeneration after injury, and fundamental epidermal developmental pathways. These studies also revealed potential effects on genes not previously implicated in ILK functions, including those important for melanocyte and melanoblast development and function, regulation of cytoskeletal dynamics, and homeobox genes. This study shows that ILK is a critical regulator of multiple ...Continue Reading

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Citations

Mar 12, 2016·The Journal of Investigative Dermatology·Samar SayedyahosseinLina Dagnino
Oct 1, 2014·The Journal of Investigative Dermatology·Linda ViLina Dagnino
Dec 29, 2013·Molecular Biology of the Cell·Alena RudkouskayaLina Dagnino
Jul 7, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Samar SayedyahosseinLina Dagnino
Mar 31, 2016·Oncotarget·Randeep K Singh, Lina Dagnino

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Methods Mentioned

BETA
Assay
in vitro transcription
reverse-transcription PCR
PCR

Software Mentioned

RMA Express
Genespring GX
Partek Genomics Suite
GSEA
LinRegPCR
Gene Set Enrichment Analysis ( GSEA )

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