PMID: 2647Feb 1, 1976

Multiple sedimenting species of properdin in human serum and interaction of purified properdin with the third component of complement

The Journal of Experimental Medicine
J Chapitis, I H Lepow

Abstract

Normal human serum subjected to sucrose density gradient analysis exhibited multiple sedimenting species of properdin antigen. Properdin antigen distribution was dependent on serum concentration, ionic strength, temperature, and the presence of C3, and was not dependent on the presence of divalent metal cations or blood coagulation. In mixtures of purified components, properdin sedimented heavier in the presence of C3, C3b, or C3c. Addition of factor B to mixtures containing C3 and properdin was without effect. These data provide insights into earlier discrepancies concerning the sedimentation behavior of partially purified properdin, indicate a propensity of some constituents of the alternative pathway to form protein-protein complexes, and suggest caution in interpretation of immunopathological studies in which properdin deposits are found in the presence of C3.

References

Jul 1, 1974·Immunochemistry·J O Minta, I H Lepow
Apr 1, 1972·The American Journal of the Medical Sciences·J Chapitis, I H Lepow
Aug 1, 1973·The Journal of Experimental Medicine·W P Kolb, H J Müller-Eberhard
Aug 1, 1973·The Journal of Experimental Medicine·W P KolbH J Müller-Eberhard
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Citations

Jul 1, 1996·Journal of Biomedical Materials Research·P TengvallI Lundström
Jan 1, 1993·Immunologic Research·K K Maves, J M Weiler
Jan 1, 1978·Journal of Immunological Methods·J O Minta, W Vetvutanapibul
Feb 1, 1978·Immunochemistry·J O Minta, W Vetvutanapibul
Nov 1, 1986·Molecular Immunology·J D Lambris, H J Müller-Eberhard
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J M WeilerD T Fearon
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D T Fearon
Jan 1, 1976·Transplantation Reviews·D T FearonK F Austen
Jul 16, 1977·British Medical Journal·M Garcia-FuentesD G Williams
Aug 1, 1978·The Journal of Clinical Investigation·M TakahashiV Nussenzweig
Jun 5, 2015·Hematology/oncology Clinics of North America·Juan Carlos Varela, Stephen Tomlinson
Apr 21, 2020·The Journal of Clinical Investigation·Eleni Gavriilaki, Robert A Brodsky
Jun 4, 2020·International Journal of Molecular Sciences·Eleni GavriilakiAchilles Anagnostopoulos

Related Concepts

Blood Group H Type 1 Antigen
Hemolytic Complement
Complement C3 precursor
Hydrogen-Ion Concentration
Lipodystrophy
Properdin
Ultracentrifugation

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