Multiple self epitopes on the Rhesus polypeptides stimulate immunologically ignorant human T cells in vitro

European Journal of Immunology
Robert N Barker, C J Elson

Abstract

The extent of autoreactive T cell repertoire in the normal individual has previously been unclear. Here we demonstrate that T cells from healthy humans can be stimulated by multiple epitopes on a self protein to give primary proliferative responses in vitro. Synthetic 15-mer peptides, corresponding to the sequence of a human red blood cell Rhesus polypeptide, were tested for the ability to stimulate normal T cells. Multiple peptides were found to provoke responses reproducibly, and the proliferation could be blocked consistently by antibodies to HLA-DR, but not -DP or -DQ. T cells from each donor proliferated in response to different patterns of peptides, but this variation in pattern was less marked in individuals with the same HLA-DR type. The responses were comparable in kinetics to those elicited by the non-recall foreign antigen keyhole limpet hemocyanin, and the responding cells are most commonly derived from the CD45RA+ subpopulation, indicating that they had not been activated in vivo. It is considered that T cells are "immunologically ignorant" of many self peptides, presumably because they correspond to cryptic epitopes that are not normally presented in vivo.

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Citations

Apr 1, 1995·Current Opinion in Immunology·J Sprent, S R Webb
Oct 3, 1999·Veterinary Immunology and Immunopathology·M J Day
Dec 5, 2000·Current Opinion in Immunology·C J Elson, R N Barker
Jun 26, 2001·Clinical and Experimental Immunology·M PonsfordD C Wraith
Jan 25, 2008·Journal of the American Society of Nephrology : JASN·Juan ZouRichard G Phelps
Feb 2, 2016·Autoimmunity·S Ju SmirnovaA B Sudarikov
Nov 7, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G DevereuxR N Barker
Jan 1, 1996·European Journal of Immunology·F E PerryC J Elson
Dec 22, 2007·Tissue Antigens·M M WaandersF H J Claas
Aug 30, 2005·Annals of the New York Academy of Sciences·Caterina Toriani-Terenzi, Enzo Fagiolo
Dec 5, 2014·Clinical and Experimental Immunology·W J PickfordA D Ormerod
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeremías GallettiMirta Giordano
Aug 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wendy J PickfordRobert N Barker
Oct 22, 2003·Journal of the American Society of Nephrology : JASN·Lindsay S CairnsRobert N Barker
Mar 28, 2006·Transfusion Medicine Reviews·Lawrence D Petz

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