PMID: 3757035Oct 10, 1986Paper

Multiple self-splicing introns in bacteriophage T4: evidence from autocatalytic GTP labeling of RNA in vitro

Cell
J M GottM Belfort

Abstract

RNA from T4-infected cells yielded multiple end-labeled species when incubated with alpha-32P-GTP under self-splicing conditions. One of these corresponds to the previously identified intron from the td gene of T4, while others appear to represent additional group I introns in T4. Two loci distinct from the td gene were found to hybridize to a mixed alpha-32P-GTP-labeled T4 RNA probe. These mapped in or near the unlinked genes nrdB and nrdC. A fragment from the nrdB region that contains the intron has been cloned and shown to generate characteristic group I splice products with RNA synthesized in vivo and in vitro. Multiple introns, and the prospect that these occur within several genes in the same metabolic pathway, suggest a possible regulatory role for splicing in T4.

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Citations

Feb 5, 1990·Journal of Molecular Biology·J L Galloway SalvoM Belfort
Jul 1, 1989·Trends in Genetics : TIG·M Belfort
Dec 1, 1991·Current Opinion in Genetics & Development·D A Shub
May 1, 1994·Nature Structural Biology·T R CechR R Gutell
Sep 14, 2004·Nature Structural & Molecular Biology·David R EdgellMarlene Belfort
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D A ShubM Belfort
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A C HuffK N Kreuzer
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F K ChuF Maley
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Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R F Doolittle
Aug 28, 2009·The Journal of Biological Chemistry·Marlene Belfort
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Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gurminder S BassiKevin M Weeks
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·V LazarevicD Karamata
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·V Derbyshire, M Belfort
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Nov 1, 1987·The Journal of protozoology·R W Finley, J A Dvorak

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