Multiple signaling conduits regulate global differentiation-specific gene expression in PC12 cells

Journal of Cellular Physiology
Lindsay MarekLynn E Heasley

Abstract

PC12 cells serve as a model for exploring nerve growth factor (NGF)-stimulated signal pathways that mediate neural differentiation. We previously demonstrated that neurofilament light chain (NFLC) gene induction by NGF requires collaborative extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) signaling. Herein, we investigate the broader requirement for integrated ERK and JNK signaling in NGF-stimulated gene expression. NGF stimulates differentiation as well as maintenance of cell viability while insulin-like growth factor-1 (IGF-1) stimulates only trophic actions in PC12 cells. Affymetrix Genechips were used to identify genes whose expression specifically increased in response to NGF, but not IGF-1. From the set of NGF-specific genes, the induction by NGF of ten genes with diverse predicted cellular functions was tested for ERK and JNK pathway requirements using the protein kinase inhibitors, PD98059 and SP600125, respectively. Like NFLC, induction of urokinase plasminogen activator (uPAR), transin/matrix metalloproteinase 3 (MMP3), Fra-1 and transforming growth factor beta 1 (TGF beta 1) required collaborative ERK and JNK signaling while the increased expression of cortexin, rat collapsin response me...Continue Reading

References

Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·F Tirone, E M Shooter
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P Masiakowski, E M Shooter
Sep 1, 1987·Molecular and Cellular Biology·D G LeonardL A Greene
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G GuroffD End
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·N H LeeA R Kerlavage
May 22, 1998·Trends in Genetics : TIG·H D Madhani, G R Fink
Sep 12, 1998·The Journal of Biological Chemistry·T Morooka, E Nishida
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·J M AngelastroL A Greene
Dec 19, 2000·The Journal of Biological Chemistry·G PearsonM H Cobb
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·B L BennettD W Anderson
Feb 2, 2002·Nature Reviews. Molecular Cell Biology·Catherine A Hazzalin, Louis C Mahadevan
Jun 21, 2002·Trends in Cell Biology·Ashton Breitkreutz, Mike Tyers
Jun 28, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Nihal Kaplan-AlbuquerqueRaphael A Nemenoff

❮ Previous
Next ❯

Citations

Jan 5, 2006·Cellular and Molecular Neurobiology·Attila BacsiIstvan Boldogh
Feb 6, 2009·Cellular and Molecular Neurobiology·Ramasamy SaminathanPonnampalam Gopalakrishnakone
Mar 5, 2008·Endocrine Pathology·James F PowersArthur S Tischler
Mar 31, 2007·Nature Neuroscience·Jian ZhongWilliam D Snider
Feb 17, 2006·Molecular Biology of the Cell·Huibin Yang, Raymond R Mattingly
Nov 30, 2005·Molecular and Cellular Biology·Claudia R AmuraLynn E Heasley
May 4, 2010·BMC Developmental Biology·Hitoshi MiyakawaToru Miura
Jul 8, 2008·Molecular and Cellular Neurosciences·Barbara L ApostolLeslie Michels Thompson
Mar 19, 2008·Journal of Neurochemistry·Thomas F DijkmansErno Vreugdenhil
Nov 22, 2007·Journal of Neurochemistry·Thomas v O HansenFinn C Nielsen
Jul 16, 2009·Hippocampus·Francesca MariniElisabetta Albi
Oct 26, 2012·Journal of Biomedicine & Biotechnology·Hongdong BaiRobert J Parmer
Aug 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ken-ichi NakajimaYoshinori Marunaka
Jan 13, 2018·Frontiers in Psychiatry·Hans C Klein
Oct 30, 2013·Endocrine-related Cancer·Ssu-Ming HuangDah-Yuu Lu
Apr 25, 2007·Journal of Neuroscience Research·Feng SuRobin Farias-Eisner
Dec 15, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Lee Clough, Leonidas Stefanis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.