PMID: 1212667Dec 1, 1975Paper

Multiple Smith-degradations of carcinoembryonic antigen (CEA) and of asialo CEA

Carbohydrate Research
E M BessellJ H Westwood

Abstract

Carcinoembryonic antigen (CEA) and asialo CEA were subjected to multiple Smith-degradation (i.e., for each degradation, application in sequence of periodate oxidation, borohydride reduction, and mild hydrolysis with acid; borohydride-t was substituted for unlabelled borohydride). High yields of modified glycoproteins were obtained at each stage. After three complete degradations and a further periodate-borohydride-t treatment, the carbohydrate content of CEA and of asialo CEA had decreased from 45-50% to 11-12% (i.e., 90% removal of carbohydrate). Glycerol was always one of the products obtained after each degradation, but threitol and erythritol were not detected. The second degradation caused a substantial loss of 2-acetamide-2-deoxyglucose, which is consistent with the location of some of this monosaccharide towards the terminal (non-reducing) end of the oligosaccharides. The "core" region of the oligosaccharides is composed of galactose, mannose, and 2-acetamido-2-deoxyglucose. After the fourth oxidation, 2-acetamido-2-deoxyglucose was 50-60% of the total content of residual carbohydrate. After the first degradation, there was a progressive loss in antigenic activity, but this was associated with a small amount of hydrolysi...Continue Reading

References

Feb 25, 1975·Biochemistry·J E Coligan, C W Todd
Sep 1, 1966·Journal of Biochemistry·M Makino, I Yamashina
Oct 1, 1970·The Biochemical Journal·B S Hartley
Jan 1, 1965·The Biochemical Journal·J R CLAMP, L HOUGH

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