Multiple specific CytR binding sites at the Escherichia coli deoP2 promoter mediate both cooperative and competitive interactions between CytR and cAMP receptor protein.

The Journal of Biological Chemistry
L T PeriniD F Senear

Abstract

Binding of cAMP receptor protein (CRP) and CytR mediates both positive and negative control of transcription from Escherichia coli deoP2. Transcription is activated by CRP and repressed by a multi-protein CRP.CytR.CRP complex. The latter is stabilized by cooperative interactions between CRP and CytR. Similar interactions at the other transcriptional units of the CytR regulon coordinate expression of the transport proteins and enzymes required for nucleoside catabolism. A fundamental question in both prokaryotic and eukaryotic gene regulation is how combinatorial mechanisms of this sort regulate differential expression. To understand the combinatorial control mechanism at deoP2, we have used quantitative footprint and gel shift analysis of CRP and CytR binding to evaluate the distribution of ligation states. By comparison to distributions for other CytR-regulated promoters, we hope to understand the roles of individual states in differential gene expression. The results indicate that CytR binds specifically to multiple sites at deoP2, including both the well recognized CytR site flanked by CRP1 and CRP2 and also sites coincident with CRP1 and CRP2. Binding to these multiple sites yields both cooperative and competitive interacti...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·D F Senear, D W Bolen
Feb 1, 1991·Molecular Microbiology·P Valentin-HansenS R Douthwaite
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L Søgaard-AndersenP Valentin-Hansen
Jul 5, 1990·European Journal of Biochemistry·A Munch-Petersen, N Jensen
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Heyduk, J C Lee
Dec 11, 1990·Nucleic Acids Research·A GunasekeraR H Ebright
Jan 1, 1988·Journal of Bacteriology·E BremerA Middendorf
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Carey
May 25, 1984·Science·B de CrombruggheH Buc
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G K AckersM A Shea
May 1, 1993·Molecular Microbiology·R H Ebright
Mar 1, 1996·Journal of Bacteriology·I BrikunD E Berg
Jan 1, 1964·Advances in Protein Chemistry·J WYMAN

❮ Previous
Next ❯

Citations

Feb 1, 1997·Current Opinion in Biotechnology·V Petri, M Brenowitz
Mar 6, 1999·Current Opinion in Microbiology·B Müller-Hill
Nov 3, 2004·Nucleic Acids Research·Dongling ZhengStephen D Minchin
Mar 4, 2005·Journal of Bacteriology·Gregory JubelinCorinne Dorel
Jun 24, 2011·Biophysical Chemistry·Keith D ConnaghanDavid L Bain
Mar 10, 2009·Current Opinion in Microbiology·Liskin Swint-Kruse, Kathleen S Matthews
Jul 4, 1997·The Journal of Biological Chemistry·C S BarbierD F Senear
Nov 8, 2012·Chembiochem : a European Journal of Chemical Biology·Maiko TanabeHirokazu Nishida

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.