Multiple splice variants of the human calcium-independent phospholipase A2 and their effect on enzyme activity.

The Journal of Biological Chemistry
P K LarssonB P Kennedy

Abstract

Recently, the cloning of a novel Ca2+-independent phospholipase A2 (iPLA2) from Chinese hamster ovary cells as well as from mouse and rat sources containing a C-terminal lipase motif and eight N-terminal ankyrin repeats has been described. In this report we describe the cloning of the human iPLA2 cDNA and its expression in B-cells and show that the iPLA2 gene undergoes extensive alternative splicing generating multiple isoforms that contribute to a novel mechanism to control iPLA2 activity. The full-length cDNA clone encodes a 806-amino acid protein with a calculated molecular mass of 88 kDa. The protein contains a lipase motif, GXSXG, and ankyrin repeats, as described for the hamster and rodent forms of the enzyme but has an additional 54-amino acid proline-rich insertion in the last of the eight ankyrin repeats (residues 395-449). Furthermore, at least three additional isoforms most likely due to alternative splicing were identified. One that is present as a partial cDNA in the expressed sequence tag data base is similar to iPLA2 but terminates just after the lipase active site, and two other isoforms contain only the iPLA2 ankyrin repeat sequence (ankyrin-iPLA2-1 and -2). Ankyrin repeats are involved in protein-protein inter...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P J JakobssonB Samuelsson
Apr 1, 1992·Trends in Biochemical Sciences·V BlankA Israël
Jul 15, 1991·Biochemical and Biophysical Research Communications·P J JakobssonH E Claesson
Nov 15, 1987·International Journal of Cancer. Journal International Du Cancer·J Avila-CariñoM G Masucci
Nov 16, 1995·Biochimica Et Biophysica Acta·E J Ackermann, E A Dennis
Aug 15, 1995·European Journal of Biochemistry·P J JakobssonH E Claesson
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·J BalsindeE A Dennis
Oct 21, 1994·Cell·Z N Oltvai, S J Korsmeyer
Dec 1, 1993·Trends in Genetics : TIG·T D Gilmore, P J Morin
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J Grumont, S Gerondakis
Jan 1, 1993·Annual Review of Cell Biology·V Bennett, D M Gilligan
Jun 28, 1996·The Journal of Biological Chemistry·D Portilla, G Dai
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·J D ClarkL L Lin
Jan 1, 1997·Trends in Biochemical Sciences·E A Dennis
Mar 28, 1997·The Journal of Biological Chemistry·J TangS S Jones
Jun 27, 1997·The Journal of Biological Chemistry·J Balsinde, E A Dennis

❮ Previous
Next ❯

Citations

Jul 27, 1999·Lipids·B ChaminadeH Chap
Oct 27, 2006·Journal of Neural Transmission·E L Schaeffer, W F Gattaz
Oct 22, 2008·Cardiovascular Drugs and Therapy·John E Burke, Edward A Dennis
Aug 12, 2006·Journal of Neurocytology·Wei-Yi OngAkhlaq A Farooqui
Jun 25, 2008·Lipids·Caroline S Beckett, Jane McHowat
Nov 19, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Brian M Ross
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Ichiro Kudo, Makoto Murakami
Mar 29, 2001·Progress in Lipid Research·E A Capper, L A Marshall
Sep 23, 2003·Brain Research. Brain Research Reviews·Michael S Lidow
Aug 21, 2003·Drug Discovery Today·Jonathan P Laye, Jason H Gill
Nov 18, 2000·Biochimica Et Biophysica Acta·M V WinsteadE A Dennis
Jul 9, 1999·Biochimica Et Biophysica Acta·S E BarbourC L Deal
Apr 28, 2006·Journal of Medicinal Chemistry·Daren StephensGeorge Kokotos
Apr 8, 2010·Journal of Medicinal Chemistry·George KokotosEdward A Dennis
Apr 17, 1999·Journal of Internal Medicine·H E Claesson, S E Dahlén
May 21, 1999·European Journal of Biochemistry·P K Larsson ForsellH E Claesson
Oct 25, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·John LindbomChrister Tagesson
Nov 5, 2008·Journal of Medical Genetics·A GregoryS J Hayflick
Dec 31, 2010·American Journal of Physiology. Cell Physiology·Janhavi SharmaJane McHowat
Apr 27, 2007·Reproductive Biology and Endocrinology : RB&E·Patricia K TithofJames D Godkin
Aug 12, 2004·Biological & Pharmaceutical Bulletin·Satoshi Akiba, Takashi Sato
Mar 20, 2008·The American Journal of Pathology·Sasanka RamanadhamJohn Turk
Sep 21, 2001·Biochemical and Biophysical Research Communications·C H Chiu, S Jackowski
Mar 10, 2011·Journal of Neural Transmission·Evelin L SchaefferWagner F Gattaz

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.