Multiple splicing events involved in regulation of human aromatase expression by a novel promoter, I.6

Endocrinology
Makio ShozuE R Simpson

Abstract

The expression of aromatase is regulated in a tissue-specific fashion through alternative use of multiple promoter-specific first exons. To date, eight different first exons have been reported in human aromatase, namely I.1., I.2, I.3. I.4, I.5, PII, 2a, and 1f. Recently, we have found a new putative exon I in a RACE-generated library of THP-1 cells and have conducted studies to characterize this new exon I. We confirmed that the constructs containing -1552/+17 or less flanking sequence of this exon function as a promoter in THP-1 cells, JEG-3 cells and osteoblast-like cells obtained from a human fetus. Results of transfection assays using a series of deletion constructs and mutation constructs indicate that a 1-bp mismatch of the consensus TATA-like box (TTTAAT) and the consensus sequence of the initiator site, which is located 45 bp downstream of the putative TATA box, were functioning cooperatively as a core promoter. The putative transcription site was confirmed by the results of RT-PCR southern blot analysis. We examined the regulation and the expression of this exon, I.6, in several human cells and tissues by RT-PCR Southern blot analysis. THP-1 cells (mononuclear leukemic origin) and JEG-3 cells (choriocarcinoma origin) ...Continue Reading

References

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Citations

Jul 21, 2011·Journal of Osteoporosis·Daniela MerlottiRanuccio Nuti
May 2, 2009·Drugs·Paolo VercelliniPier Giorgio Crosignani
Oct 29, 2008·Reproductive Sciences·Masashi DemuraSerdar E Bulun
Aug 12, 1999·International Journal of Andrology·S CarreauJ Levallet
Apr 1, 2005·International Journal of Cancer. Journal International Du Cancer·Randi A PaynterImmaculata De Vivo
Dec 9, 2008·Acta Pharmacologica Sinica·Heng-hui ChengQiu-rong Ruan
Jul 26, 2006·Expert Opinion on Drug Metabolism & Toxicology·Cheri M TurmanHenry W Strobel
Sep 30, 2014·Expert Review of Endocrinology & Metabolism·Makio ShozuTsutomu Ogata
Apr 5, 2007·The Journal of Clinical Endocrinology and Metabolism·María Sonia BaquedanoAlicia Belgorosky
Jan 3, 2001·The Journal of Clinical Endocrinology and Metabolism·T OkuboS Chen
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Serge Carreau, Jérôme Levallet
Jun 15, 2016·Neural Plasticity·Janine Prange-KielGabriele M Rune
Mar 1, 2018·Hormone Molecular Biology and Clinical Investigation·Keigo MurakamiHironobu Sasano

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