Multiple states of a nucleotide-bound group 2 chaperonin.

Structure
Daniel K ClareHelen R Saibil

Abstract

Chaperonin action is controlled by cycles of nucleotide binding and hydrolysis. Here, we examine the effects of nucleotide binding on an archaeal group 2 chaperonin. In contrast to the ordered apo state of the group 1 chaperonin GroEL, the unliganded form of the homo-16-mer Methanococcus maripaludis group 2 chaperonin is very open and flexible, with intersubunit contacts only in the central double belt of equatorial domains. The intermediate and apical domains are free of contacts and deviate significantly from the overall 8-fold symmetry. Nucleotide binding results in three distinct, ordered 8-fold symmetric conformations--open, partially closed, and fully closed. The partially closed ring encloses a 40% larger volume than does the GroEL-GroES folding chamber, enabling it to encapsulate proteins up to 80 kDa, in contrast to the fully closed form, whose cavities are 20% smaller than those of the GroEL-GroES chamber.

References

Jan 1, 1996·Journal of Structural Biology·R A CrowtherJ M Smith
Jan 1, 1996·Journal of Structural Biology·M van HeelM Schatz
Feb 26, 1998·Cell·B Bukau, A L Horwich
Oct 6, 1998·Annual Review of Biochemistry·P B SiglerA L Horwich
Mar 27, 2001·Protein Science : a Publication of the Protein Society·G KafriA Horovitz
Oct 3, 2001·Journal of Structural Biology·I GutscheR P May
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·J NavazaJ Borge
Jun 5, 2003·Journal of Structural Biology·Joseph A Mindell, Nikolaus Grigorieff
Nov 3, 2004·Trends in Cell Biology·Christoph SpiessJudith Frydman
Apr 6, 2005·Journal of Molecular Biology·Maria Giulia Bigotti, Anthony R Clarke
May 14, 2005·Journal of Structural Biology·Christian SulowayBridget Carragher
Oct 27, 2005·Current Opinion in Structural Biology·Amnon Horovitz, Keith R Willison
May 11, 2006·The Journal of Biological Chemistry·Matthew J CliffAnthony R Clarke
May 11, 2007·Annual Review of Cell and Developmental Biology·Arthur L HorwichGeorge W Farr
May 15, 2007·Molecular Cell·Nadav EladHelen R Saibil

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Citations

Jun 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Junjie ZhangMichael Levitt
Sep 13, 2013·Nature Reviews. Molecular Cell Biology·Helen Saibil
Aug 20, 2010·Journal of Structural Biology·Michael StölkenStephan Nickell
Feb 19, 2009·Current Opinion in Cell Biology·Daniel RusselAndrej Sali
Dec 24, 2011·The EMBO Journal·Jose H PereiraPaul D Adams
Oct 29, 2010·Protein Science : a Publication of the Protein Society·Kelly M KneeJonathan A King
Jul 24, 2013·Biopolymers·Daniel K Clare, Helen R Saibil
Mar 30, 2016·Structure·Daniel Hoersch, Tanja Kortemme
Jan 27, 2011·Biochemical Society Transactions·Peter Lund
Feb 13, 2015·Bioinformatics·Elihu C Ihms, Mark P Foster
Sep 4, 2013·Journal of Structural Biology·José Ramón Lopéz-Blanco, Pablo Chacón
Oct 16, 2010·Structure·Yanwu HuoFei Sun
Aug 14, 2010·Molecular Cell·Matthias P Mayer
Nov 1, 2016·Nature Structural & Molecular Biology·Yunxiang ZangYao Cong
Mar 23, 2018·International Journal of Molecular Sciences·Naoki OgawaMasafumi Yohda
Dec 14, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Deborah K ShoemarkMaria Giulia Bigotti
Jan 22, 2010·Nature·Junjie ZhangWah Chiu

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