Multiple stem cell traits of expanded rat bone marrow stromal cells

Experimental Neurology
Jung Yeon LimSeok-Gu Kang

Abstract

Bone marrow stromal cells (BMSC) exhibit many traits of a stem cell population. Knowing that BMSC have the ability to self-renew, proliferate and differentiate into a variety of cell types, questions may arise as to whether these traits differ between the cells that have different expansion times. In this study, we examined the stem cell potentiality of BMSC through their characterization, proliferative capacity and the ability to differentiate into multiple lineages in the cultured 2nd passage cells and 10th passage cells. The results were as follows: (1) the 10th passage cells had a larger and more flatted morphology than the 2nd passage cells and also exhibited a decreased labeling for BMSC-related antigens such as CD90, CD73. (2) The cell proliferative capacity was approximately 2 times greater in the 2nd passage cells, and the apoptosis phenomenon was detected in the 10th passage cells. (3) The ability to differentiate into mesodermal tissue (osteocytes, adipocytes), as well as into ectodermal tissue (neurons) was more effective in the 2nd passage cells. Taken together, early stage BMSC would be a valuable cell source for various in vitro applications, as well as cell therapy.

References

Nov 24, 1995·Science·Z XiaM E Greenberg
Apr 4, 1997·Science·R McKay
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A Eglitis, E Mezey
Aug 1, 1997·The Journal of Biological Chemistry·J D English, J D Sweatt
Apr 2, 1999·Science·M F PittengerD R Marshak
Apr 10, 1999·Nature Neuroscience·C M AtkinsJ D Sweatt
Jun 11, 1999·Neuroreport·M A EglitisM M Mouradian
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·G C KopenD G Phinney
Aug 1, 2000·Experimental Neurology·J Sanchez-RamosP R Sanberg
Sep 20, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Y LiZ Zhang
Aug 3, 2001·Blood Cells, Molecules & Diseases·I B Black, D Woodbury
Nov 22, 2001·Biochemical and Biophysical Research Communications·F BarryJ Zaia
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·C P HofstetterL Olson
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Jan 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eva MezeyBarbara Crain
Feb 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·James M WeimannHelen M Blau
Oct 23, 2003·Neuropathology : Official Journal of the Japanese Society of Neuropathology·JangBo LeeYoshinobu Iwasaki
Dec 16, 2003·Birth Defects Research. Part C, Embryo Today : Reviews·Frank P Barry
Feb 26, 2004·Experimental Cell Research·Javorina MilosevicJohannes Schwarz
May 21, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sean M GallagherKimberly M Huber
Jun 24, 2004·Journal of Neuroscience Research·Paul LuMark H Tuszynski

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Citations

Feb 9, 2008·Archives of Dermatological Research·Paolo SbanoRiccardo Saccardi
Apr 24, 2010·Tissue Engineering. Part a·Abigail M WojtowiczAndrés J García
Jul 4, 2012·Brain Research·Hae-Young Lopilly ParkChan Kee Park
Apr 30, 2010·Differentiation; Research in Biological Diversity·Jun LiLanjuan Li
Jan 31, 2009·Cell Transplantation·Wen Yu LiOh Young Bang

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