PMID: 8938130Nov 1, 1996Paper

Multiple structural elements in voltage-dependent Ca2+ channels support their inhibition by G proteins

Neuron
J F ZhangR W Tsien

Abstract

Molecular determinants of Ca2+ channel responsiveness to inhibition by receptor-coupled G proteins were investigated in Xenopus oocytes. The inhibitory response of alpha1B (N-type) channels was much larger than alpha1A (P/Q-type) channels, while alpha1C (L-type) channels were unresponsive. Differences in both degree and speed of inhibition were accounted for by variations in inhibitor off-rate. We tested proposals that inhibitory G protein and Ca2+ channel beta subunits compete specifically at the I-II loop. G protein-mediated inhibition remained unaltered in alpha1B subunits containing a point mutation in the I-II loop segment critical for Ca2+ channel beta subunit binding, and in chimeras where the I-II loop of alpha1B was replaced with counterparts from alpha1A or alpha1c. Full interconversion between modulatory behaviors of alpha1B and alpha1A was achieved only by swapping both motif I and the C-terminus in combination. Thus, essential structural elements for G protein modulation reside in multiple Ca2+ channel domains.

References

Jun 1, 1992·Current Opinion in Neurobiology·T P Snutch, P B Reiner
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H Kasai
Sep 1, 1991·Brain Research. Brain Research Reviews·R Anwyl
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R A GrossR L Macdonald
Feb 1, 1986·Pflügers Archiv : European journal of physiology·C MarchettiH D Lux
Oct 1, 1988·Trends in Neurosciences·R W TsienA P Fox
Feb 1, 1989·The Journal of Physiology·S R Ikeda, G G Schofield
Jun 1, 1989·Pflügers Archiv : European journal of physiology·H Kasai, T Aosaki
Jan 1, 1989·Progress in Biophysics and Molecular Biology·E Carbone, D Swandulla
Nov 1, 1967·The Journal of Physiology·F A Dodge, R Rahamimoff
Nov 24, 1995·Science·A TaddeseE W McCleskey
Dec 1, 1994·Trends in Neurosciences·B Hille
Mar 17, 1995·Biochemical and Biophysical Research Communications·J ZhouT Tanabe
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·J NakaiK G Beam

❮ Previous
Next ❯

Citations

Jul 17, 2003·Progress in Biophysics and Molecular Biology·Stefan Offermanns
Apr 29, 1998·Trends in Neurosciences·D Walker, M De Waard
Sep 6, 2002·Neuroscience Letters·Carmelo Millán, José Sánchez-Prieto
Oct 12, 2001·Trends in Endocrinology and Metabolism : TEM·N Dascal
Feb 2, 2000·The Journal of General Physiology·H M ColecraftD T Yue
Jul 13, 2011·Cold Spring Harbor Perspectives in Biology·William A Catterall
Nov 21, 2007·Acta Physiologica·A P FoxZ Xie
Oct 14, 2000·Annual Review of Cell and Developmental Biology·W A Catterall
May 23, 1998·Annual Review of Pharmacology and Toxicology·R J Miller
Dec 26, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yun ZhangJian Yang
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M JiangL Birnbaumer
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Guillaume SandozMichel De Waard
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·G W Zamponi, T P Snutch
Dec 16, 2006·Pflügers Archiv : European journal of physiology·Norbert WeissMichel De Waard
Nov 24, 1999·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·K Gardner
Apr 12, 2003·The Bulletin of Tokyo Dental College·Takayuki EndohTakashi Suzuki
Mar 3, 1998·The Journal of Physiology·A C Dolphin
Sep 10, 2003·The Journal of Physiology·Masahiro KimuraTomoyuki Takahashi
Dec 4, 2015·Brain Sciences·Edgar Garcia-RillCristy Phillips
Dec 17, 2011·Neurobiology of Disease·Leonardo C FariaDavid A Prince
Jun 17, 1998·The Journal of Physiology·G J StephensA C Dolphin
Jun 17, 1998·The Journal of Physiology·G J StephensA C Dolphin
Jul 23, 2005·The Journal of Physiology·Taro IshikawaTomoyuki Takahashi
Jan 24, 2007·Journal of Neurochemistry·Douglas P OlsenMichele H Jacob
Oct 20, 2007·Journal of Neurochemistry·Carolina LaderaJosé Sánchez-Prieto
Apr 7, 2004·British Journal of Pharmacology·Silvana De LorenzoAdriana Losavio
Oct 16, 2012·Biochimica Et Biophysica Acta·Gerald W Zamponi, Kevin P M Currie

❮ Previous
Next ❯

Related Concepts

Related Feeds

BRAIN Initiative Cell Census Network (BICCN)

The BRAIN Initiative Cell Census Network aims to identify and provide experimental access to the different brain cell types to determine their roles in health and disease. Discover the latest research from researchers in the BRAIN Initiative Cell Census Network here.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.