Multiple subtypes of phospholipase C are encoded by the norpA gene of Drosophila melanogaster.

The Journal of Biological Chemistry
S KimR D Shortridge

Abstract

The norpA gene of Drosophila melanogaster encodes a phosphatidylinositol-specific phospholipase C that is essential for phototransduction. Besides being found abundantly in retina, norpA gene products are expressed in a variety of tissues that do not contain phototransduction machinery, implying that norpA is involved in signaling pathways in addition to phototransduction. We have identified a second subtype of norpA protein that is generated by alternative splicing of norpA RNA. The alternative splicing occurs at a single exon that is excluded from mature norpA transcripts when a substitute exon of equal size is retained. The net difference between the two subtypes of norpA protein is 14 amino acid substitutions occurring between amino acid positions 130 and 155 of the enzyme. Results from Northern analyses suggest that norpA subtype I transcripts are most abundantly expressed in adult retina, while subtype II transcripts are most abundant in adult body. Moreover, norpA subtype I RNA can be detected by the reverse transcription-polymerase chain reaction in extracts of adult head tissue but not adult body nor at earlier stages of Drosophila development. Conversely, norpA subtype II RNA can be detected by reverse transcription-p...Continue Reading

References

Dec 15, 1992·The Biochemical Journal·B K Drøbak
Jan 1, 1992·Progress in Growth Factor Research·G Jones, G Carpenter
Nov 1, 1991·Trends in Neurosciences·R RanganathanC S Zuker
Mar 19, 1991·Biochimica Et Biophysica Acta·E MeldrumA Carozzi
Mar 1, 1990·The American Review of Respiratory Disease·P D Lew
Feb 1, 1989·Biochemical Society Transactions·R H Michell
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S H RyuS G Rhee
Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·Y Hotta, S Benzer
Aug 1, 1970·Nature·W L PakK S Arnold
Jul 11, 1984·Nucleic Acids Research·P O O'Connell, M Rosbash
Jun 1, 1980·Behavioral and Neural Biology·T A Markow, M Manning
May 1, 1982·Behavior Genetics·L TompkinsR W Siegel
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J Riesgo-EscovarJ R Carlson
Jun 2, 1995·The Journal of Biological Chemistry·R R McKayR D Shortridge
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P A FerreiraW L Pak
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D WuM I Simon

❮ Previous
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Citations

Dec 1, 1995·Invertebrate Neuroscience : in·R D Shortridge, R R McKay
May 10, 2007·Pflügers Archiv : European journal of physiology·Tao Wang, Craig Montell
Sep 6, 2000·British Journal of Haematology·G F MaoA Koneti Rao
Nov 9, 2000·The European Journal of Neuroscience·A HuberR Paulsen
Nov 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ujjaini DasguptaUsha Acharya
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J Riesgo-EscovarJ R Carlson
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·C E AlvarezW Gilbert
Jul 30, 2009·Journal of Lipid Research·Raju V S Rajala
Feb 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·B H CollinsC P Kyriacou
Mar 26, 2003·Insect Molecular Biology·S KimR D Shortridge
Jan 31, 2012·Journal of Neurogenetics·Randall D Shortridge
Jun 16, 2001·Journal of Biological Rhythms·M IvanchenkoJ M Giebultowicz
Mar 7, 1998·The Journal of Biological Chemistry·M J KimP G Suh
Oct 4, 2000·Physiological Reviews·M J Rebecchi, S N Pentyala
Jun 21, 2020·Biology·Carlo BredaCharalambos P Kyriacou
Jan 23, 1999·The Journal of Biological Chemistry·K NaganoT Takenawa
Jan 5, 1996·The Journal of Biological Chemistry·S B Lee, S G Rhee
Oct 14, 2011·The Journal of Experimental Biology·Markus FriedrichStewart B Peck

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