PMID: 11914130Mar 27, 2002Paper

Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae

BMC Genetics
W Shou, R J Deshaies

Abstract

The Mitotic Exit Network (MEN) proteins - including the protein kinase Cdc15 and the protein phosphatase Cdc14 - are essential for exit from mitosis in Saccharomyces cerevisiae. To identify downstream targets of the MEN, we sought telophase arrest bypassed (tab) mutations that bypassed the essential requirement for CDC15. Previous studies identified net1(tab2-1) and CDC14(TAB6-1) as mutations in the RENT complex subunits Net1 and Cdc14, respectively, and revealed that the MEN acts by promoting release of Cdc14 from its nucleolar Net1 anchor during anaphase. However, the remaining tab mutants were not characterized. Fourteen out of fifteen tab mutants were mapped to three recessive (tab1-tab3) and three dominant (TAB5-TAB7) linkage groups. We show that net1(tab2-1) enables growth of tem1Delta, cdc15Delta, dbf2Delta dbf20Delta, and mob1Delta, but not cdc5Delta or cdc14Delta, arguing that whereas the essential task of the first four genes is to promote exit from mitosis, CDC5 possesses additional essential function(s). net1(tab2-1) but not CDC14(TAB6-1) resulted in a high rate of chromosome loss, indicating that Net1 promotes accurate chromosome segregation in addition to its multiple known roles. Finally, TAB1 was shown to be MTR...Continue Reading

Citations

Jun 1, 2007·Molecular Biology of the Cell·Quan-Wen JinDannel McCollum
Nov 3, 2010·The Journal of Biological Chemistry·Ying-Chou Chen, Michael Weinreich
Jul 31, 2007·Genetics·M Evangelina Pablo-HernandoCarlos R Vázquez de Aldana
Jan 16, 2009·Molecular Biology of the Cell·Ying Lu, Frederick Cross
Dec 18, 2013·PLoS Genetics·Barbara BaroEthel Queralt
Nov 18, 2003·Annual Review of Genetics·Daniel J Lew, Daniel J Burke
Dec 1, 2004·Annual Review of Genetics·Frank Stegmeier, Angelika Amon
Feb 18, 2009·The Journal of Cell Biology·Dane A MohlRaymond J Deshaies
Dec 10, 2015·NPJ Systems Biology and Applications·Pavel KraikivskiJohn J Tyson
Nov 21, 2018·Nature Structural & Molecular Biology·Meghna KatariaFrank Uhlmann

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