Multiple transcription factors regulate the inducible expression of the human complement receptor 2 promoter

The Journal of Immunology : Official Journal of the American Association of Immunologists
Lyudmila A VereshchaginaGeorge C Tsokos

Abstract

Complement receptor 2 (CR2) is regulated at the transcriptional level, but the promoter elements and the transcription factors that bind to them and contribute to its regulation are unknown. After documenting that PMA and cAMP induced the activity of the CR2 promoter by 10-fold, we conducted promoter truncation and mutagenesis experiments, in conjunction with shift assays, to determine the functionally important regions of the promoter and the proteins that bind to them. We identified two regions, separated by approximately 900 nucleotides, which together were responsible for inducible promoter activity. Mutagenesis of single promoter elements demonstrated a functional upstream stimulatory factor/E box in the TATA box-proximal region and three equally important, closely spaced, CREB/AP-1 half-sites in the upstream promoter region. The cAMP response element-binding protein (CREB)/AP-1 half-sites bound in vitro Jun and CREB that are induced by protein kinases A and/or C. The 900-nucleotide segment stretching between the above two regions had no functional impact on the induced transcription, and its deletion increased the promoter activity. Finally, a region upstream of the distal site had a repressor activity on CR2 transcriptio...Continue Reading

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Citations

Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniela UlgiatiV Michael Holers
Oct 6, 2007·Immunologic Research·Brian K Martin
Apr 11, 2008·Clinical and Vaccine Immunology : CVI·Dong LiuZhong-Xiang Niu
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mate TolnayGeorge C Tsokos
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irina DebnathJohn H Weis
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hui WuBetty P Tsao
Sep 23, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Susan A Boackle
Sep 4, 2004·Biochemical Pharmacology·Ziad Abdel-RazzakRobert Barouki

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