Multiple transcripts of a gene for a leucine-rich repeat receptor kinase from morning glory (Ipomoea nil) originate from different TATA boxes in a tissue-specific manner

Molecular Genetics and Genomics : MGG
Carole L BassettM Harrison

Abstract

TATA boxes are the most common regulatory elements found in the promoters of eukaryotic genes because they are associated with basal transcription initiation by RNA polymerase II. Often only a single TATA element is found in a given promoter, and tissue-, stage- and/or stimulus-specific expression occurs because the TATA box is associated with other cis -acting elements that enhance or repress transcription. We used software tools for gene analysis to assist in locating potential TATA box(es) in an AT-rich region of the promoter of a gene, inrpk1, which codes for a leucine-rich receptor protein kinase in morning glory (Ipomoea nil). Through the use of RT-PCR and various combinations of forward primers bracketing most of the promoter region we were able to define the 5'-ends of transcripts in this region. The region was then targeted for analysis by RNA Ligase-Mediated-5' Rapid Amplification of cDNA Ends (RLM-5' RACE) to identify the transcript initiation site(s). Positioning of initiation sites with respect to TATA boxes identified by gene analysis tools allowed us to identify three operational TATA elements which regulate basal transcription from this gene. Two TATA boxes were responsible for all of the inrpk1 transcripts foun...Continue Reading

References

Apr 1, 1991·Computer Applications in the Biosciences : CABIOS·D S Prestridge
Apr 7, 1989·Cell·S T Smale, D Baltimore
Jan 1, 1989·Molecular & General Genetics : MGG·D GidoniP Dunsmuir
Jun 1, 1995·The Plant Journal : for Cell and Molecular Biology·M TamaokiM Matsuoka
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R D Damiani, S R Wessler
Oct 1, 1995·Computer Applications in the Biosciences : CABIOS·Y V KondrakhinL Milanesi
Oct 1, 1996·Computer Applications in the Biosciences : CABIOS·L MilanesiP Arrigo
Jan 14, 1998·Plant Molecular Biology·C P JoshiV L Chiang
Nov 14, 2000·Molecular and Cellular Biology·D R Morris, A P Geballe
Jan 29, 2002·Nucleic Acids Research·M Kozak
Apr 18, 2002·Current Opinion in Structural Biology·Stephen K Burley, Katsuhiko Kamada
Oct 9, 2002·Molecular and Cellular Biology·Baoguang Han, Jian-Ting Zhang
Jun 26, 2003·Nucleic Acids Research·A E KelE Wingender
Feb 1, 1972·Plant Physiology·K C Short, J G Torrey

❮ Previous
Next ❯

Citations

Mar 14, 2012·The Plant Journal : for Cell and Molecular Biology·Saminathan ThangasamyGuang-Yuh Jauh
Mar 4, 2015·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Rosa M MorelosAna M Ibarra
Dec 21, 2006·Molecular and Cellular Biochemistry·Haijing LiuBo Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.